Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives

ISBN-13: 9781493958474 / Angielski / Miękka / 2016 / 409 str.

Jonathan M. Gibbins; Martyn P. Mahaut-Smith

Opis

This comprehensive volume covers techniques for the study of platelet function, approaches for investigating megakaryocyte function, and perspectives on important overall concepts in the field of megakaryocyte and platelet biology.

Spis treści

Part I: Additional Protocols for the Study of Platelet Function

 

1. Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally-Induced Vascular Damage and Plaque Rupture In Vivo

            Marijke J.E. Kuijpers and Johan W.M. Heemskerk

 

2. Assessment of Platelet Aggregation Responses In Vivo in the Mouse

            Christopher Moore and Michael Emerson

 

3. Focal Cerebral Ischemia

            Stefan Braeuninger, Christoph Kleinschnitz, Bernhard Nieswandt, and Guido Stoll

 

4. Extracorporeal Assays of Thrombosis

            Lina Badimon, Teresa Padro,  and Gemma Vilahur

 

5. Platelet Life Span and Apoptosis

            Emma C. Josefsson, Michael J. White, Mark R. Dowling, and Benjamin T. Kile

 

6. A Live Cell Micro-Imaging Technique to Examine Platelet Calcium Signalling Dynamics Under Blood Flow

            Warwick S. Nesbitt, Ian S. Harper, Simone M. Schoenwaelder, Yuping Yuan, and Shaun P. Jackson

 

7. Platelet Shape Change and Spreading

            Joseph E. Aslan, Asako Itakura, Jacqueline M. Gertz, and Owen J.T. McCarty

 

8. Clot Retraction

            Katherine L. Tucker, Tanya Sage, and Jonathan M. Gibbins

 

9. Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton

            Jonathan N. Thon and Joseph E. Italiano

 

10. Measurement of Platelet Microparticles

            Jeffrey I. Zwicker, Romaric Lacroix, Françoise Dignat-George, Barbara C. Furie, and Bruce Furie

 

11. Assessing Protein Synthesis by Platelets

            Hansjörg Schwertz, Jesse W. Rowley, Neal D. Tolley, Robert A. Campbell, and Andrew S. Weyrich

 

12. A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies

            Stefan Amisten

 

Part II: Additional Protocols for the Study of Megakaryocyte Function

 

13. Characterization of Megakaryocyte Development in the Native Bone Marrow Environment

            Anita Eckly, Catherine Strassel, Jean-Pierre Cazenave, François Lanza, Catherine Léon, and Christian Gachet

 

14. Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow

            Harald Schulze

 

15. In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

            Naoya Takayama and Koji Eto

 

16. Megakaryocyte and Platelet Production from Human Cord Blood Stem Cells

            Amélie Robert, Valérie Cortin, Alain Garnier, and Nicolas Pineault

 

17. Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Line

            Yumiko Matsubara, Mitsuru Murata, and Yasuo Ikeda

 

18. Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression

            Gwen Tolhurst, Richard N. Carter, Nigel Miller, and Martyn P. Mahaut-Smith

 

19. Assessment of Megakaryocyte Migration and Chemotaxis

            Alexandra Mazharian

 

Part III: Perspectives on the Study of Platelet and Megakaryocyte Function

 

20. Megakaryopoiesis and Thrombopoiesis: An Update on Cytokines and Lineage Surface Markers

            Ming Yu and Alan B. Cantor

 

21. Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation

            Christopher M. Williams and Alastair W. Poole

 

22. Platelet Receptor Shedding

            Elizabeth E. Gardiner, Mohammad Al-Tamimi, Robert K. Andrews, and Michael C. Berndt

 

23. Endogenous Inhibitory Mechanisms and the Regulation of Platelet Function

            Chris I. Jones, Natasha E. Barrett, Leonardo A. Moraes, Jonathan M. Gibbins, and Denise E. Jackson

 

24. Platelet Proteomics: State of the Art and Future Perspective

            Yotis Senis and Ángel García

Blurb

New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established. Thus, the main aim of Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives is to complement the first two volumes published in 2004 by adding recently developed state of the art techniques.  Conveniently divided into three sections, this detailed volume covers techniques to study platelet function, approaches to investigate megakaryocyte function, and perspectives on important overall concepts in the field of megakaryocyte and platelet biology.  Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.