Plasmodesmata: Methods and Protocols

ISBN-13: 9781071621318 / Angielski / Twarda / 2022 / 486 str.

Benitez-Alfonso, Yoselin

Opis

This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways. 

Spis treści

Part I: Overview Articles

 

1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development

            Philip Kirk and Yoselin Benitez-Alfonso

 

2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses

            Caiping Huang and Manfred Heinlein

 

Part II: Structure and Distribution of Plasmodesmata

 

3. Plasmodesmata Ultrastructure Determination Using Electron Tomography

            Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer

 

4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata

            Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska

 

5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots

            Andrea Paterlini and Ilya Belevich

 

6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities

            Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith

 

7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy

            Florence R. Danila

 

8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy

            Kirsten Knox

 

Part III: Compositional Analysis of Plasmodesmata

 

9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata

            Caiping Huang, Jerôme Mutterer, and Manfred Heinlein

 

10. Immunofluorescence Detection of Callose in Plant Tissue Sections

            Sam Amsbury and Yoselin Benitez-Alfonso

 

11. Callose Detection and Quantification at Plasmodesmata in Bryophytes

            Arthur Muller, Tomomichi Fujita, and Yoan Coudert

 

12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis

            Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison

 

13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons

            Verena Kriechbaumer and Stanley W. Botchway

 

14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer

            Patrick Blümke, Vicky Howe, and Rüdiger Simon

 

15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy

            Joseph F. McKenna

 

16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering

            Ajeet Chaudhary and Kay Schneitz

 

Part IV: Functional Analysis of Plasmodesmata

 

17. Quantification of Cell-to-Cell Connectivity Using Particle Bombardment

            Estee E. Tee, Sebastian Samwald, and Christine Faulkner

 

18. Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay

            Jessica C. Fernandez and Tessa M. Burch-Smith

 

19. Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay

            Wilson Horner and Jacob O. Brunkard

 

20. An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins

            Frank Machin, Yağmur Hasbioğlu, and Friedrich Kragler

 

21. Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP

            Min Diao and Shanjin Huang

 

22. Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes

            Takumi Tomoi, Yoan Coudert, and Tomomichi Fujita

 

23. Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability

            Ekaterina A. Lazareva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergey Y. Morozov, Manfred Heinlein, and Andrey G. Solovyev

 

24. Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis

            Justyna Wróbel-Marek, Kamila Godel-Jędrychowska, and Ewa Kurczyńska

 

25. Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling

            Lisa Van den Broeck, Mariah Gobble, and Rosangela Sozzani

 

26. Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction

            Dawei Yan

 

27. A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue

            Munenori Kitagawa and David Jackson

 

Part V: Other Approaches Contributing to Plasmodesmata Research

 

28. In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG

            Eduardo J. Peña and Manfred Heinlein

 

29. Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM)

            Michael Fuchs and Jan U. Lohmann

 

30. More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight

            Eva E. Deinum

 

31. Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis

            Gwendolyn V. Davis, Richard S. Smith, and George W. Bassel