Plant-Virus Interactions

ISBN-13: 9781071634844 / Angielski / Twarda / 2023

Opis

This detailed volume provides practical guidance on techniques in plant-virus interaction research, from targeting specific molecular interactions within the virus-host interactome to the identification of the complete virus-host protein-protein interaction network. After chapters on acquiring the necessary molecular tools, the book continues with biochemical and genetic approaches to confirming protein-protein interactions both in vivo and in vitro, procedures and protocols for assessing replication, translation, viral genome movement, and insect transmission, as well as techniques for detecting multiple molecular interactions between the host and the virus and monitoring immune hubs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Plant-Virus Interactions serves as a valuable resource for understanding the protein-protein interaction network between the virus and the host, crucial for comprehending the life cycle of a virus and for developing strategies for broad-spectrum and long-lasting resistance against viral infections.

Spis treści

1. Computational Pipeline for Detection of Plant RNA Viruses Using High-Throughput Sequencing

            Livia Donaire and Miguel A. Aranda

 

2. Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons

            João Paulo Herrera da Silva and F. Murilo Zerbini

 

3. Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral cDNAs (icDNAs)

            Maija Pollari

 

4. Construction of Geminivirus Infectious Clones for Agroinoculation into Plants

            Ivair J. Morais, Alice K. Inoue-Nagata, and Erich Y.T. Nakasu

 

5. Agroinoculation of Geminiviral Infectious Clones into Plants

            Ivair J. Morais, Yanca F. M. Ferreira, Erich Y.T. Nakasu, Tatsuya Nagata, and Alice K. Inoue-Nagata

 

6. Begomovirus Inoculation in Arabidopsis and Cassava

            José T. Ascencio-Ibáñez, Mary M. Dallas, and Linda Hanley-Bowdoin

 

7. Absolute and Relative Quantification of Single-Stranded Positive Sense RNA Virus from Plant Tissue

            Pinky Dutta and Kristiina Mäkinen

 

8. Quantification of Virion-Sense and Complementary-Sense DNA Strands of Circular Single-Stranded DNA Viruses

            Edgar A. Rodríguez-Negrete and Ana Grande-Pérez

 

9. Replication Assay of Begomovirus in Arabidopsis Protoplasts

            Laura Gonçalves Costa Martins and Elizabeth P.B. Fontes

 

10. Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye

            Yinzi Li and Aiming Wang

 

11. Ribosome Profiling of Plants

            Jahanara Sonia, Pulkit Kanodia, Zachary Lozier, and W. Allen Miller

 

12. Experimental Transmission of Plant Viruses by Aphids or Whiteflies

            Irene Ontiveros, Juan Antonio Diaz-Pendón, and Juan José López-Moya

 

13. Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana

            Miriam Gil-Valle, Cristina Sáez, Nuria Montes, and Israel Pagán

 

14. Protein-Protein Interaction via Two-Hybrid Assay in Yeast

            Christiane Eliza Motta Duarte and Nivea Costa Euclydes

 

15. Bimolecular Fluorescence Complementation (BIFC) in Host-Virus Interactions

            Fredy Davi Albuquerque Silva, João Paulo Batista Machado, and Pedro Augusto Braga dos Reis

 

16. Co-Immunoprecipitation Assays to Detect Protein-Protein Interactions

            Mengling Huang, Xiao Yu, and Bo Li

 

17. Split-Luciferase Complementation Imaging Assay in Virus-Plant Interactions

            Huang Tan, Xi Zhang, and Rosa Lozano-Duran

 

18. The Split-Luciferase Complementation Assay to Detect and Quantify Protein-Protein Interactions In Planta

            Eduardo Bassi Simoni and Célio Cabral Oliveira

 

19. TurboID-Based Proximity Labeling: A Method to Decipher Protein-Protein Interactions in Plants

            Yuanyuan Li, Yongliang Zhang, and Savithramma P. Dinesh-Kumar

 

20. Virus-Induced Heritable Gene Editing in Plants

            Ugrappa Nagalakshmi, Nathan Meier, and Savithramma P. Dinesh-Kumar

 

21. Immunoprecipitation Followed by Mass Spectrometry: An Approach for Identifying Host-Viral Protein-Protein Interactions

            Laura Medina-Puche and Rosa Lozano-Duran

 

22. In Situ Protein Microarray for Identifying the Geminivirus-Arabidopsis Interactome

            Junshi Yazaki and Maximiller Dal-Bianco