Plant Senescence: Methods and Protocols

ISBN-13: 9781493985401 / Angielski / Miękka / 2019 / 367 str.

Yongfeng Guo

Spis treści

Part I: Introduction of Plant Senescence

 

1. Concepts and Types of Senescence in Plants

            Susheng Gan

 

2. Initiation, Progression, and Genetic Manipulation of Leaf Senescence

            Akhtar Ali, Xiaoming Gao, and Yongfeng Guo

 

Part II: Phenotypic Analysis and Molecular Markers of Plant Organ Senescence

 

3. Phenotypic Analysis and Molecular Markers of Leaf Senescence

            Liming Zhao, Yan Xia, Xiao-Yuan Wu, Jos H.M. Schippers, and Hai-Chun Jing

 

4. Investigation of Petal Senescence by TRV-Mediated Virus-Induced Gene Silencing in Rose

            Chenxia Cheng, Junping Gao, and Nan Ma

 

5. Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence

            Xiuying Xia

 

6. Quantitative Analysis of Floral Organ Abscission in Arabidopsis via a Petal Breakstrength Assay

            Chun-Lin Shi and Melinka A. Butenko

 

7. Characterization of Climacteric and Non-Climacteric Fruit Ripening

            Xiaohong Kou and Mengshi Wu

 

Part III: Hormonal Control of Plant Senescence

 

8. Ethylene Treatment in Studying Leaf Senescence in Arabidopsis

            Zhonghai Li and Hongwei Guo

 

9. The Assay of Abscisic Acid-Induced Stomata Movement in Leaf Senescence

            Yanyan Zhang and Kewei Zhang

 

10. The EPR Method for Detecting Nitric Oxide in Plant Senescence

            Aizhen Sun

 

11. Hormone Treatments in Studying Leaf Senescence

            Zenglin Zhang and Yongfeng Guo

 

Part IV: Stress-Induced Senescence in Plants

 

12. Methods to Study Darkness-Induced Leaf Senescence

            Yi Song and Lin Li

 

13. Salt Treatments and Induction of Senescence

            Yasuhito Sakuraba, Dami Kim, and Nam-Chon Paek

 

14. Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance

            Shoki Aoyama, Junji Yamaguchi, and Takeo Sato

 

15. Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection

            Wei Liu, Wenwei Zhang, Na Zheng, Weibo Zhai, and Fangjun Qi

 

16. Study of Hydrogen Peroxide as a Senescence-Inducing Signal

            Stefan Bieker, Maren Potschin, and Ulrike Zentgraf

 

17. Identification of Postharvest Senescence Regulators through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue

            Donald A. Hunter, Rubina Jibran, Paul Dijkwel, David Chagné, Kerry Sullivan, Aakansha Kanojia, and Ross Crowhurst

 

Part V: Molecular and Cellular Processes in Plant Senescence

 

18. Chlorophyll and Chlorophyll Catabolite Analysis by HPLC

            Aditi Das, Luzia Guyer, and Stefan Hörtensteiner

 

19. Identification and Functional Analysis of Senescence-Associated Genes in Wheat

            Geng Wang, Ke Li, and Chunjiang Zhou

 

20. Isolation, Purification, and Detection of MicroRNAs in Plant Senescence

            Yujun Ren and Ying Miao

 

21. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay

            Branka Uzelac, Dušica Janošević, and Snežana Budimir

 

22. Activities of Vacuolar Cysteine Proteases in Plant Senescence

            Dana E Martínez, Lorenza Costa, and Juan José Guiamét

 

23. Study of Autophagy in Plant Senescence

            Xuefei Cui, Jing Zheng, Jinxin Zheng, and Qingqiu Gong

 

24. Isolation of Chloroplasts for In Organelle Protein Degradation Assay

            Scott W. Vande Wetering and Judy A. Brusslan

 

25. Plant Cell Walls: Isolation and Monosaccharide Composition Analysis

            Yingzhen Kong, Malcolm O’Neill, and Gongke Zhou

 

26. Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope

            Chun-Lin Shi and Melinka A. Butenko

 

Part VI: Systems Biology in Plant Senescence

 

27. RNA-Seq Analysis of the Transcriptome of Leaf Senescence in Tobacco

            Wei Li and Yongfeng Guo

 

28. Comprehensive Metabolomics Studies of Plant Developmental Senescence

            Mutsumi Watanabe, Takayuki Tohge, Salma Balazadeh, Alexander Erban, Patrick Giavalisco, Joachim Kopka, Bernd Mueller-Roeber, Alisdair R. Fernie, and Rainer Hoefgen

Blurb

This detailed volume covers a wide variety of techniques either developed specially for plant senescence studies or optimized for studying senescing plants. After an introduction to the topic, the book continues with sections on phenotypic analysis and molecular markers of plant organ senescence, hormonal control of plant senescence, stress-induced senescence, molecular and cellular processes in plant senescence, as well as systems biology approaches. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Senescence: Methods and Protocols aims to provide a useful hand book of standard protocols for plant molecular biologists working on senescence.