Plant Respiration and Internal Oxygen: Methods and Protocols

ISBN-13: 9781493972913 / Angielski / Twarda / 2017 / 285 str.

Kapuganti Jagadis Gupta

Spis treści

1. Assessing Metabolic Flux in Plants with Radiorespirometry

Nicholas J. Kruger, Shyam K. Masakapalli, and R. George Ratcliffe

 

2. Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory Fluxes

Toshihiro Obata, Laise Rosado-Souza, and Alisdair R. Fernie

 

3. A Method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution

 Hardy Rolletschek and Gregor Liebsch

 

4. VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots

Aprajita Kumari and Kapuganti Jagadis Gupta

 

5. MultiSense: A Multimodal Sensor Tool Enabling the High Throughput Analysis of Respiration

Peter Keil, Gregor Liebsch, Ljudmilla Borisjuk, and Hardy Rolletschek

 

6. Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds using Micro Sensor

Sonika Pandey, Aprajita Kumari, Bharadwaj C, and Kapuganti Jagadis Gupta

 

7. Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringe Infection

Pradeep Kumar Pathak and Kapuganti Jagadis Gupta

 

8. Measurement of Oxygen Status in Arabidopsis Leaves Undergoing Hypersensitive Response during Pseudomonas Infection

Aprajita Kumari, Gail Preston, and Kapuganti Jagadis Gupta

 

9. Isolation of Physiologically Active and Intact Mitochondria from Chickpea

Sonika Pandey, Aprajita Kumari, and Kapuganti Jagadis Gupta

 

10. Isolation and Structural Studies of Mitochondria from Pea Roots

 Abhaypratap Vishwakarma and Kapuganti Jagadis Gupta

 

11. Mitochondrial Respiration and Oxygen Tension

Daniel S. Shaw, Karlia Meitha, Michael J. Considine, and Christine H. Foyer

 

12. Isolation of Mitochondria from Model and Crop Plants

Sandra M. Kerbler and Nicolas L. Taylor

 

13. Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in Fruits

Jurandi Gonçalves de Oliveira, Luis Miguel Mazorra Morales, Gláucia Michelle Cosme Silva, Kátia Daniella da Cruz   Saraiva, Diederson Bortolino Santana, Clesivan Pereira dos Santos, Marcos Goes Oliveira, and José Hélio Costa

 

14. Measurement of Tricarboxilic Acid Cycle Enzyme Activities in Plants

 Rebeca Patricia Omena-Garcia, Wagner L. Araújo, Yves Gibon, Alisdair R. Fernie, and Adriano Nunes-Nesi

 

15. Respiration Traits as Novel Markers for Plant Robustness under the Threat of Climate Change – A   Protocol for Validation

Birgit Arnholdt-Schmitt

 

16. Calorespirometry – A Novel Tool in Functional Hologenomics to Select ‘Green’ Holobionts for Biomass Production

Birgit Arnholdt-Schmitt and Vinod Kumar Patil

 

17. Measurements of Electron Partitioning Between Cytochrome and Alternative Oxidase Pathways in Plant Tissues

Nestor Fernandez Del-Saz, Miquel Ribas-Carbo, Gabriel Martorell, Alisdair R. Fernie, and Igor Florez-Sarasa

 

18. A Driving Bioinformatics Approach to Explore Co-regulation of AOX Gene Family Members during Growth and Development

José Hélio Costa and Birgit Arnholdt-Schmitt

 

19. A Step-by-Step Protocol for Classifying AOX Proteins in Flowering Plants

José Hélio Costa, Clesivan Pereira dos Santos, Kátia Daniella da Cruz Saraiva, and Birgit Arnholdt-Schmitt

 

20. Studying Individual Plant AOX Gene Functionality in Early Growth Regulation – A New Approach

Birgit Arnholdt-Schmitt and Vinod Kumar Patil

 

21. Laser-Capture Microdissection for Amplification of Alternative Oxidase (AOX) Genes in Target Tissues in Daucus carota L.

Carla Ragonezi and Birgit Arnholdt-Schmitt

 

22. Measurement of Mitochondrial Respiration in Isolated Protoplasts: Cytochrome and Alternative Pathways

Bobba Sunil and Agepati S. Raghavendra

 

23. Measuring Spatial and Temporal Oxygen Flux near Plant Tissues using a Self-Referencing Optrode

Eric S. McLamore, D.M. Porterfield, and Yinglang Wan

Blurb

This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation techniques, protocols for dual-inlet isotope ratio mass spectrometry, laser-capture microdissection, and bioinformatics approach for exploring the co-regulation of AOX gene family members. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Respiration and Internal Oxygen: Methods and Protocols aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen.