Plant Protoplasts and Genetic Engineering IV

ISBN-13: 9783642780394 / Angielski / Miękka / 2011 / 390 str.

Opis

In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.

Spis treści

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Kiwifruit (Actinidia deliciosa).- 1 Introduction.- 2 Isolation and Culture of Protoplasts.- 3 Growth of Regenerated Plants.- 4 Variability in Regenerated Plants.- 5 Conclusions and Prospects.- References.- I.2 Regeneration of Plants from Protoplasts of Duboisia.- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Fusion of Protoplasts.- 6 Miscellaneous Study.- 7 Conclusion and Prospects.- 8 Protocol.- References.- I.3 Regeneration of Plants from Protoplasts of Cultivated Strawberry (Fragaria x ananassa) and Wild Strawberry (Fragaria vesca).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Characterization of Protoplast-Derived Plants.- 6 Genetic Transformation in Strawberry.- 7 Conclusions and Prospects.- 8 Protocol.- References.- I.4 Regeneration of Plants from Protoplasts of Sweet Potato (Ipomoea batatas L. Lam.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Characterization of Protoplast-Derived Plants.- 6 Fusion of Protoplasts.- 7 Summary and Conclusion.- 8 Protocol.- References.- I.5 Regeneration of Plants from Alfalfa (Medicago sativa L.) Protoplasts by Direct Embryogenesis.- 1 Introduction.- 2 Procedures.- 3 Results.- 4 Discussion.- 5 Protocol.- References.- I.6 Regeneration of Plants from Protoplasts of Adsuki Bean (Phaseolus angularis Wight).- 1 Introduction.- 2 Material and Methods.- 3 Results and Discussion.- 4 Summary.- References.- I.7 Regeneration of Plants from Protoplasts of Some Stone Fruits (Prunus spp.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Protoplast Fusion and Somatic Hybridization Studies with Prunus Genotypes.- 6 Miscellaneous Studies with Prunus Protoplasts.- 7 Conclusions.- 8 References.- I.8 Regeneration of Plants from Protoplasts of Winged Bean (Psophocarpus tetragonolobus L. DC.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Protoplast Purification.- 4 Protoplast Culture.- 5 Plant Regeneration from Protoclones.- 6 Conclusion.- 7 Protocol.- References.- I.9 Regeneration of Plants from Protoplasts of Eggplant (Solanum melongena L.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Fusion of Protoplasts.- 6 Summary and Conclusion.- 7 Protocol.- References.- I.10 Regeneration of Plants from Protoplasts of Sorghum (Sorghum vulgare).- 1 Introduction.- 2 Embryogénie Callus Formation and Establishment of Suspension Culture.- 3 Protoplast Isolation and Culture.- 4 Plant Regeneration.- 5 Protoplast Transformation by PEG-Mediated DNA Transfer.- 6 Conclusion.- 7 Protocol.- References.- Section II Genetic Transformation.- II. 1 Transformation in Horseradish (Armoracia rusticana): Hairy Roots Incited by Agrobacterium rhizogenes Infection.- 1 Introduction.- 2 Varieties of Horseradish.- 3 Induction of Hairy Roots.- 3 Confirmation of Ri Plasmid-Direction Transformation.- 5 Growth of Hairy Roots.- 6 Bioreactor for Hairy Root Culture.- 7 Peroxidase Productivity of Hairy Roots.- 8 Regeneration of Hairy Roots.- 9 Summary and Conclusion.- References.- II.2 Transformation in Sugar Beet (Beta vulgaris L.).- 1 Introduction.- 2 Transformation by Direct Gene Transfer.- 3 Transformation by Agrobacterium tumefaciens.- 4 Conclusions.- References.- II.3 Transformation in Oilseed Rape (Brassica napus L.).- 1 Introduction.- 2 Susceptibility of Brassica napus to Agrobacterium tumefaciens Infection.- 3 Transformation of Tissue Explants.- 4 Transformation of Protoplasts.- 5 Gene Transfer and Regeneration Protocol.- 6 Summary and Conclusion.- 7 Culture Media and Solutions.- References.- II.4 Transformation in Chickpea (Cicer arietinum L.).- 1 Introduction.- 2 Agrobacterium Chickpea Interaction.- 3 Analysis of Transformed Tissues.- 4 Summary and Conclusions.- References.- II.5 Transformation in Citrus.- 1 Introduction.- 2 Review of Previous Research on Citrus Transformation.- 3 Transformation Parameters.- 4 Evaluation of Transformation.- 5 Conclusions and Prospects.- 6 Protocol.- References.- II.6 Transformation in Muskmelon (Cucumis melo L.).- 1 Introduction.- 2 A. tumefaciens-Mediated Transformation and Regeneration of Muskmelon Plants.- 3 Summary and Conclusions.- References.- 11.7 Transformation in Cucumber (Cucumis sativus L.).- 1 Introduction.- 2 Materials and Methods.- 3 Experimental Results.- 4 Preliminary Field Testing of Plants Which Express the CMV-C Coat Protein Gene.- 5 Second Field Testing of Transgenic Cucumber Plants That Express the CMV Coat Protein Gene.- 6 Conclusion.- References.- II.8 Transformation in Soybean (Glycine max L.).- 1 Introduction.- 2 Delivery of DNA into Soybean Cells.- 3 Regeneration of Transformed Soybeans.- 4 Conclusions and Future Prospects.- References.- II.9 Transformation of Lettuce (Lactuca sativa L.).- 1 General Account.- 2 Transformation in Lactuca sativa L.- 3 Inoculation of Agrobacterium and Selection of Transformants.- 4 GUS Assay and Kanamycin Resistance in the Self-Pollinated Progeny.- 5 Histochemical Localization of Salicylic Acid and Bacterial Stress-Induced GUS Activity.- 6 Southern Blot Analysis.- 7 Conclusions and Prospects.- 8 Protocol.- References.- II. 10 Transformation of Birds-Foot Trefoil (Lotus corniculatus).- 1 Introduction.- 2 Transformation of Lotus corniculatus.- 3 An Overview of Genetic Transformation of Forage Legumes.- 4 Concluding Remarks.- 5 Protocol.- References.- II.11 Transformation of Tomato (Lycopersicon esculentum Mill.) for Virus Disease Protection.- 1 Introduction.- 2 Review of Transformation Work in Tomato.- 3 Intranuclear Microinjection for Transformation of Tomato Callus Cells.- 4 Transformation of Tomato with Mutagenized Coat Protein (MCP) Gene.- 5 Summary and Conclusion.- References.- II.12 Transformation in Cassava (Manihot esculenta Crantz).- 1 Introduction.- 2 Experiments with a Particle Gun: Transient Expression of the GUS Gene.- 3 Transformation of Leaf Disks with Agrobacterium tumefaciens.- 4 Transformation of Leaf Lobes by Bombardment with an Agrobacterium tumefaciens Suspension.- 5 Transformation of Somatic Embryos with Agrobacterium tumefaciens.- 6 Antibiotics and Selection of Transformed Tissues.- 7 Summary and Conclusions.- References.- II.13 Transformation of Tobacco (Nicotiana clevelandii and N. benthamiana).- 1 Introduction.- 2 Transformation Studies on Nicotiana.- 3 Transformation of Nicotiana clevelandii and N. benthamiana.- 4 Prospects and Conclusions.- References.- II.14 Transformation of Petunia and Corn Plants (Petunia hybrida and Zea mays) Using Agrobacterium tumefaciens and the Shoot Ape.- 1 Introduction.- 2 Methods.- 3 Results.- 4 Discussion.- 5 Assumptions.- 6 Conclusions.- References.- II.15 Transformation in Spruce (Picea Species).- 1 Introduction.- 2 Gene Transfer Systems.- 3 Selection.- 4 Gene Expression.- 5 Regeneration of Transformed White Spruce.- 6 Summary.- References.- 11.16 Transformation of Pea (Pisum sativum L.).- 1 Introduction.- 2 Pea Tissue Culture/Regeneration in Vitro.- 3 Transformation.- 4 Concluding Remarks.- References.- II.17 Transformation of Sugarcane.- 1 Why Genetic Engineering Is Attractive for Sugarcane Variety Improvement.- 2 Sugarcane Tissue Culture.- 3 Gene Transfer Techniques.- 4 Promoters and Marker Genes for Sugarcane Transformation.- 5 Prospects.- References.- II.18 Transformation of Stylosanthes Species.- 1 Introduction.- 2 Tissue and Protoplast Culture of Stylosanthes spp.- 3 Agrobacterium-Mediated Transformation.- 4 Transient Gene Expression in S. humilis Using DNA-Coated Microprojectiles.- 5 Selection of Transformed Tissues.- 6 Reporter Gene Expression in Stably Transformed Tissues.- 7 Genes for Improvement.- 8 Summary.- References.- II.19 Transformation in Grapevine (Vitis spp.).- 1 Introduction.- 2 Genetic Transformation of Grape.- 3 Conclusions and Future Prospects.- References.