Plant-Pathogen Interactions

ISBN-13: 9781071631584 / Angielski

Nora A. Foroud; Jonathan A. D. Neilson

Opis

This detailed collection focuses on current methodologies for studying various crop diseases. The book explores phytopathogen detection, host-pathogen interactions at the earliest stages of colonization, RNA-seq analyses, as well as host responses downstream of, or coincidental to, gene expression and a newly established protocol for gene editing of Medicago sativa. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant-Pathogen Interactions serves as an ideal guide for researchers working in the vital area of plant diseases.

Spis treści

1. Specific Detection and Quantification of Major Fusarium spp. Associated with Cereal and Pulse Crops

            Mohamed Hafez, Melissa Telfer, Syama Chatterton, and Reem Aboukhaddour

 

2. Distinguishing Puccinia striiformis f. sp. tritici Isolates Using Genomic Sequencing: A Case Study

            Michele Frick, Eric Amundsen, and André Laroche

 

3. DNA-Barcoding Identification of Plant Pathogens for Disease Diagnostics

            Nicolas Feau, Padmini Herath, and Richard C. Hamelin

 

4. Real-Time Portable LAMP Assay for a Rapid Detection of Xylella fastidiosa in Field

            Nicola Luchi, Duccio Migliorini, Francesco Pecori, and Alberto Santini

 

5. Selective Quantification of Chemotropic Responses of Fusarium graminearum

            Pooja S. Sridhar, Tanya Sharma, and Michele C. Loewen

 

6. Live Cell Visualization of Early Stages of Root Colonization by the Vascular Wilt Pathogen Fusarium oxysporum

            Amey Redkar, Antonio Di Pietro, and David Turrà

 

7. Transfection of Barley Leaf Protoplasts with a Fluorescently-Tagged Fungal Effector for In Planta Localization Studies in Barley

            Ana Priscilla Montenegro Alonso and Guus Bakkeren

 

8. A Bioinformatic Guide to Identify Protein Effectors from Phytopathogens

            Christopher Blackman and Rajagopal Subramaniam

 

9. Unraveling Plant-Pathogen Interactions in Cereals Using RNA-Seq

            Bronwyn E. Rowland, Maria Antonia Henriquez, Kirby T. Nilsen, Rajagopal Subramaniam, and Sean Walkowiak

 

10. RNA-Seq Data Processing in Plant-Pathogen Interaction System: A Case Study

            Ziying Liu, Youlian Pan, Yifeng Li, Therese Ouellet, and Nora A. Foroud

 

11. Differential Expression Feature Extraction (DEFE): A Case Study in Wheat FHB RNA-Seq Data Analysis

            Youlian Pan, Anuradha Surendra, Ziying Liu, Thérèse Ouellet, and Nora A. Foroud

 

12. Proteomic Profiling of Host Response in the Cereal Crop Triticum aestivum to the Mycotoxin, 15-Acetyldeoxynivalenol, Produced by the Fungal Pathogen, Fusarium graminearum

            Reid Buchanan, Mitra Serajazari, and Jennifer Geddes-McAlister

 

13. Quantitative Phosphoproteome Analysis of the Interaction between Fusarium graminearum and Triticum aestivum

            Boyan Liu, Danisha Johal, Reid Buchanan, Brianna Ball, Mitra Serajazari, and Jennifer Geddes-McAlister

 

14. Fatty Acid Profiling of Grapevine Extracellular Compartment

            Ana Rita Cavaco, Gonçalo Laureano, Andreia Figueiredo, and Ana Rita Matos

 

15. Identifying Fungal Secondary Metabolites and Their Role in Plant Pathogenesis

            Joanna Tannous, Jesse Labbé, and Nancy P. Keller

 

16. Eliciting Targeted Mutations in Medicago sativa Using CRISPR/Cas9-Mediated Genome Editing: A Potential Tool for the Improvement of Disease Resistance

            Udaya Subedi, Kimberley Burton Hughes, Guanqun Chen, Abdelali Hannoufa, and Stacy D. Singer