Plant Nitric Oxide: Methods and Protocols

ISBN-13: 9781493935987 / Angielski / Twarda / 2016 / 203 str.

Kapuganti Jagadis Gupta

Opis

With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research.

Spis treści

1. An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods?

            Hideo Yamasaki, Naoko S. Watanabe, Yasuko Sakihama, and Michael F. Cohen

 

2. Chemiluminescence Detection of Nitric Oxide from Roots, Leaves, and Root Mitochondria

            Aakanksha Wany, Alok K . Gupta, , Aprajita Kumari,  Shika Gupta, Sonal Mishra, Ritika Jaintu, Pradeep K. Phatak, and Kapuganti Jagadis Gupta

 

3. Nitric Oxide Measurement from Purified Enzymes and Estimation of Scavenging Activity by Gas Phase Chemlimunimescence Method

            Aprajita Kumari, Alok K. Gupta, Sonal Mishra, Aakanksha Wany, and Kapuganti Jagadis Gupta

 

4. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence

            Aakanksha Wany and Kapuganti Jagadis Gupta

 

5. Nitric Oxide (NO) Measurements in Stomatal Guard Cells

            Srinivas Agurla, Gunja Gayatri, and Agepati S. Raghavendra

 

6. Methods to Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO?

            Stefan Ruemer, Markus Krischke, Agnes Fekete, Maria Lesch, Marin J. Mueller, and Werner M. Kaiser

 

7. A Novel Protocol for Detection of Nitric Oxide in Plants

            Prachi Jain and Satish C. Bhatla

 

8. Detection of Nitric Oxide by Electron Paramagnetic Resonance Spectroscopy: Spin-Trapping with Iron-Dithiocarbamates

            Luisa B. Maia and José J.G. Moura

 

9. Measurement of Nitric Oxide (NO) Generation Rate by Chloroplasts Employing Electron Spin Resonance (ESR)

            Andrea Galatro and Susana Puntarulo

 

10. Laser-Based Methods for Detection of Nitric Oxide in Plants

            Julien Mandon, Luis A.J. Mur, Frans J.M. Harren, and Simona M. Cristescu

 

11. Electrochemical Detection of Nitric Oxide in Plant Cell Suspensions

            Sophie Griveau, Angélique Besson-Bard, Fethi Bedioui, and David Wendehenne

 

12. Quantification and Localization of S-Nitrosothiols (SNOs) in Higher Plants

            Juan B. Barroso, Raquel Valderrama, Alfonso Carreras, Mounira Chaki, Juan C. Begara-Morales, Beatriz Sánchez-Calvo, and Francisco J. Corpas

 

13. Analysis of the Expression and Activity of Nitric Oxide Synthase from Marine Photosynthetic Microorganisms

            Foresi Noelia, Correa Aragunde Natalia, Santolini Jerome, and Lamattina Lorenzo

 

14. Identification of S-Nitrosothiols by the Sequential Cysteine Blocking Technique

            Rafael A. Homem, Thierry Le Bihan, Manda Yu, and Gary J. Loake

 

15. Detection of S-Nitrosoglutathione Reductase Activity in Plants

            Lucie Kubienová, Tereza Tichá, Lenka Luhová, and Marek Petřivalský

 

16. Detection of Peroxynitrite in Plants Exposed to Bacterial Infection

            Diana Bellin, Massimo Delledonne, and Elodie Vandelle