Plant Molecular Biology Manual

ISBN-13: 9789401069182 / Angielski / Miękka / 2011 / 492 str.

Stanton Gelvin

Opis

Recognizing the rapidly changing nature of plant molecular biology technology, the editors have designed a laboratory manual that is both easy to use in the laboratory and which will be updated as the techniques change and new technologies are devised.

Spis treści

Section A: Introduction of DNA into Plant Cells.- 1. Direct DNA transfer to protoplasts with and without electroporation.- 2. Use of cointegrating Ti plasmid vectors.- 3. Binary vectors.- 4. Agrobacterium molecular genetics.- 5. Leaf disc transformation.- 6. Extraction of DNA from plant tissues.- 7. Procedures for contructing ds-cDNA clone banks.- 8. Procedures for constructing genomic clone banks.- 9. Selectable and screenable markers.- 10. Transformation of plant pathogenic fungi.- Section B: Expression of Genes in Plants.- 1. Use of reporter genes to study gene expression in plant cells.- 2. Assays for studying chromatin structure.- 3. Assays for studying DNA methylation.- 4. Analysis of gene expression in transgenic plants.- 5. Subcellular targeting of proteins in vivo and in vitro.- 6. Isolation of total and polysomal RNA from plant tissues.- 7. Translation in Xenopus oocytes of mRNAs transcribed in vitro.- 8. Use of the firefly luciferase gene as a reporter of gene expression in plants.- 9. In situ RNA hybridization in plant tissues.- 10. Light-regulated promoter sequences in the multigene Cab and rbcS gene families.- 11. In vitro DNA footprinting.- 12. In vivo detection of protein-DNA interactions.- Section C: Fate of Introduced Genes.- 1. Stability of introduced genes and stability in expression.- 2. Restriction fragment length polymorphism.