Plant Mitochondria: Methods and Protocols

ISBN-13: 9781493926381 / Angielski / Twarda / 2015 / 303 str.

James Whelan; Monika W. Murcha

Opis

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.

Spis treści

1. Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa

            Monika W. Murcha and James Whelan

 

2. Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5’ End Maturation

            Stefan Binder and Kristina Kühn

 

3. Mitochondrial Run-On Transcription Assay Using Biotin Labeling

            Kristina Kühn

 

4. In Vitro RNA Uptake Studies in Plant Mitochondria

            Szymon Kubiszewski-Jakubiak, Cyrille Megel, Elodie Ubrig, Thalia Salinas, Anne-Marie Duchêne, and Laurence Maréchal-Drouard

 

5. In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria

            Owen Duncan, Chris Carrie, Yan Wang, and Monika W. Murcha

 

6. Plant Mitochondrial Proteomics

            Nicolas L. Taylor and A. Harvey Millar

 

7. Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites

            Markus Hartl, Ann-Christine König, and Iris Finkemeier

 

8. A Flowchart to Analyze Protease Activity in Plant Mitochondria

            Pedro F. Teixeira, Rui M. Branca, Beata Kmiec, and Elzbieta Glaser

 

9. Activity Measurements of Mitochondrial Enzymes in Native Gels

            Peter Schertl and Hans-Peter Braun

 

10. Activity Assay for Plant Mitochondrial Enzymes

            Shaobai Huang, Chun Pong Lee, and A. Harvey Millar

 

11. Analysis of Type II NAD(P)H Dehydrogenases

            Kathleen L. Soole and Chevaun A. Smith

 

12. Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes

            Richard P. Jacoby, A. Harvey Millar, and Nicolas L. Taylor

 

13. Micro-Respiration Measurements in Plants

            Yun Shin Sew, A. Harvey Millar, and Elke Stroeher

 

14. Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation

            Richard Fly, James Lloyd, Stephan Krueger, Alisdair Fernie, and Margaretha J. van der Merwe

 

15. Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana

            Theresa J. Reape, Joanna Kacprzyk, Niall Brogan, Lee Sweetlove, and Paul F. McCabe

 

16. Imaging and Analysis of Mitochondrial Dynamics in Living Cells

            Sanjaya B. Ekanayake, Amr M. El Zawily, Gaël Paszkiewicz, Aurélia Rolland, and David C. Logan

 

17. Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors

            Stephan Wagner, Thomas Nietzel, Isabel Aller, Alex Costa, Mark D. Fricker, Andreas J. Meyer, and Markus Schwarzländer

 

18. In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide

            Marina Cvetkovska and Greg C. Vanlerberghe

 

19. Databases and Informatics Resources For Analysis of Plant Mitochondria

            Reena Narsai

 

20. Expression and Crystallization of the Plant Alternative Oxidase

            Benjamin May, Catherine Elliott, Momi Iwata, Luke Young, Julia Shearman, Mary S. Albury, and Anthony L. Moore