Plant Mineral Nutrients: Methods and Protocols

ISBN-13: 9781627031516 / Angielski / Twarda / 2012 / 297 str.

Frans Maathuis

Opis

The study of plant mineral nutrition has both academic and applied aspects to it. Today, research into plant mineral nutrition is more pertinent than ever in the face of a growing world population and the increasing need for sustainable agriculture. In "Plant Mineral Nutrients: Methods and Protocols, "expert researchers in the field detail a comprehensive collection of methodologies that are routinely used in plant mineral nutrition research. These include methods and protocols for plant growth parameters, ion contents and composition, soil analyses, flux measurements and the use of public facilities for high throughput analyses. Written in the highly successful "Methods in Molecular Biology " series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, "Plant Mineral Nutrients: Methods and Protocols "seeks to aid scientist in the further study of into plants and their mineral nutrients.

Spis treści

1. Roles and Functions of Plant Mineral Nutrients

Frans J.M. Maathuis and Eugene Diatloff

 

2. Plant Growth and Cultivation

Dorina Podar

 

3. Protocols for Growing Plant Symbioses; Mycorrhiza

Michael Schultze

 

4. Protocols For Growing Plant Symbioses; Hizobia

Benjamin Gourion, Marie Bourcy, Viviane Cosson and Pascal Ratet

 

5. Plant Cell Suspension Cultures

Roberto Moscatiello, Barbara Baldan, and Lorella Navazio

 

6. Soil Analysis using Visible and Near Infrared Spectroscopy

Johanna Wetterlind, Bo Stenberg and Raphael A. Viscarra Rossel

 

7. Mineral Composition Analysis: Measuring Anion Uptake and Anion Concentrations in Plant Tissues

Malcolm J. Hawkesford, Saroj Parmar and Peter Buchner

 

8. Multi-element Plant Tissue Analysis using ICP Spectrometry

T.H. Hansen, T.C. de Bang, K.H. Laursen, P. Pedas, S. Husted and J.K. Schjoerring

 

9. Mapping Element Distributions in Plant Tissues using Synchrotron X-ray Fluorescence Techniques

Erica Donner, Martin D. de Jonge, Peter M. Kopittke, and Enzo Lombi

 

10. Flux Measurements of Cations using Radioactive Tracers

D.T. Britto and H.J. Kronzucker

 

11. Ion Flux Measurements using the MIFE Technique

Sergey Shabala, Lana Shabala, Jayakumar Bose, Tracey Cuin, and Ian Newman

 

12. Sampling and Analysis of Phloem Sap

Sylvie Dinant and Julia Kehr

 

13. Methods for Xylem Sap Collection

Alexou M. and Peuke A.D

 

 14. Plant Single Cell Sampling

Wieland Fricke

 

15. Measurements of Cytosolic Ion Concentrations in Live Cells

Veena S Anil, Kavitha P G, Sam Kuruvilla, Pavan Kumar and M K Mathew

 

16. Real Time Measurement of Cytoplasmic Ions with Ion Selective Microelectrodes

Anthony J Miller

 

17. Large Scale Plant Ionomics

John M. C. Danku, Brett Lahner, Elena Yakubova, and David E. Salt

 

18. Applications of High-throughput Plant Phenotyping to Study Nutrient use Efficiency

Bettina Berger, Bas de Regt, and Mark Tester