Plant Metabolomics: Methods and Protocols

ISBN-13: 9781493957484 / Angielski / Miękka / 2016 / 340 str.

Nigel W. Hardy; Robert D. Hall

Opis

Estimating the metabolite complement of plant material involves a wide, and growing, range of techniques. This up-to-date guide provides stepwise, reproducible methodologies as well as key notes and implementation advice from experts in the field.

Recenzje

From the reviews:

"The chapters presented in this volume stay true to the Methods and Protocols series, with a short piece of background information, a highly detailed step-by-step protocol, and additional practical information that will allow beginners in the field to set up repeatable, high-quality metabolomics experiments. ... this is an excellent addition to the series and can provide muchneeded support, especially to scientists starting in the growing field of plant metabolomics." (Alexandre Foito, Annals of Botany, Vol. 113 (7), April, 2014)

"The book 'Plant Metabolomics. Methods and Protocols' is fresh in ideas and a useful manual for scientists, researchers, scholars, students and the wider readership interested in plant metabolism and metabolite molecules. ... The purpose of this volume is to provide information on basic practical questions concerning experimental work with metabolomics. ... The book is a mix between a scientific treatise and a laboratory manual. As such it is an excellent one." (Tadeusz Aniszewski, Plant Science Bulletin, Vol. 58 (4), 2012)

Spis treści

1. Practical Applications of Metabolomics in Plant Biology

Robert D. Hall and Nigel Hardy

Material Preparation

1. Aspects of Experimental Design for Plant Metabolomics Experiments and Guidelines for Growth of Plant Material

Yves Gibon and Dominique Rolin

 

1. Separating the Inseparable: The Metabolomic Analysis of Plant – Pathogen Interactions

J. William Allwood , Jim Heald , Amanda J. Lloyd , Royston Goodacre  and Luis A. J. Mur

 

1. Precautions for Harvest, Sampling, Storage and Transport of Crop Plant Metabolomics Samples

 

Benoît Biais, Stéphane Bernillon, Catherine Deborde, Cécile Cabasson, Dominique Rolin, Yaakov Tadmor, Joseph Burger, Arthur A. Schaffer, Annick Moing

 

1. Tissue Preparation using Arabidopsis

Aimee M. Galster, Jennie Lewis, Sonia J. Miller, Delia-Irina Corol, Michael H. Beale and Jane L. Ward

 

Chemical Analysis Approaches

1. Solid Phase Micro-Extraction (SPME) GC - MS Analysis of Natural Volatile Components in Melon and Rice

Harrie A.  Verhoeven, Harry Jonker, Ric C.H. de Vosand Robert D Hall

 

1. Profiling Primary Metabolites of Tomato Fruit with Gas Chromatography/Mass-Spectrometry

Sonia Osorio, Phuc Thi Do and Alisdair R. Fernie

 

1. High Performance Liquid Chromatography (HPLC)- Mass Spectrometry (MS) Analysis of Plant Metabolites in Brassicaceae

Ric C.H. De Vos, Bert Schipper  and Robert D. Hall

 

1. UPLC-MS-based Metabolite Analysis in Tomato

Ilana Rogachev and Asaph Aharoni

 

1. High Precision Measurement and Fragmentation Analysis for Metabolite Identification

Madalina Oppermann, Nicolaie Eugen Damoc, Catharina Crone, Thomas Moehring, Helmut Muenster, Martin Hornshaw

 

1. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) for Plant Metabolite Profiling and Metabolite Identification

J. William Allwood, David Parker, Manfred Beckmann , John Draper , Royston Goodacre

 

1. Combined NMR and Flow Injection ESI-MS for Brassicaceae Metabolomics

National Centre for Plant and Combined NMR and Flow Injection ESI-MS for Brassicaceae Metabolomics

John M. Baker, Jane L. Ward and Michael H. Beale

 

1. ICP-MS and LC-ICP-MS for Analysis of Trace Element Content and Speciation in the Cereal Grain

Persson, D.P., Hansen, T.H., Laursen, K.H., Husted, S. and Schjoerring, J.K.

 

1. The Use of Genomics and Metabolomics Methods to Quantify Fungal Endosymbionts and Alkaloids in Grasses

Susanne Rasmussen, Geoffrey A. Lane, Wade Mace, Anthony J. Parsons and Hong Xue

 

 

Data Analysis

1. Data (pre-)processing of Nominal and Accurate Mass LC-MS or GC-MS Data using MetAlign

A.    Lommen

1. TagFinder-Preprocessing Software for the Fingerprinting and the Profiling of Gas Chromatography-Mass Spectrometry

(GC-MS) Based Metabolome Analyses

Alexander Luedemann, Luise von Malotky, Alexander Erban, Joachim Kopka

 

 

1. Chemical Identification Strategies using Liquid Chromatography-photo Diode Array-solid Phase Extraction-nuclear Magnetic Resonance / Mass Spectrometry

Sofia Moco and Jacques Vervoort

 

1. A Strategy for Selecting Data Mining Techniques in Metabolomics

Ahmed Hmaidan BaniMustafa, Nigel William Hardy

Blurb

Estimation of the metabolite complement of plant material involves a wide range of techniques and technologies and that breadth continues to increase.  Metabolomics research typically involves multiple sites for material preparation and analysis and most investigations are “high throughput”, meaning that chemical analysis of sample sets are inevitably carried out over an extended period of time.  In, Plant Metabolomics: Methods and Protocols expert researchers in the field detail many of the stages which are now commonly used to study plant metabolomics workflow. Stages of this workflow, up to and including the statistical analysis, accurate and detailed collection of meta-data are also essential for good process management, to satisfy reporting requirements and to ensure wider interpretability and reuse  results.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

  

  

Through and intuitive Plant Metabolomics: Methods and Protocols,  seeks to aid scientists in the further study of the methods for all the stages of the plant metabolomics workflow.