Plant Meiosis: Methods and Protocols

ISBN-13: 9781493998173 / Angielski / Twarda / 2019 / 405 str.

Monica Pradillo; Stefan Heckmann

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Cytological and Imaging Approaches to Study Arabidopsis Meiosis

1. A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis

Maria Ada Prusicki, Yuki Hamamura, and Arp Schnittger 

2. Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes

Santiago Valentín Galvan Gordillo, Rocio Escobar-Guzman, Daniel Rodriguez-Leal, Jean-Philippe Vielle-Calzada, and Arnaud Ronceret

3. How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana

Pablo Parra-Nunez, Mónica Pradillo, and Juan Luis Santos

4. Cytological Characterization of Arabidopsis arenosa Polyploids by SIM

Chris Morgan and Eva Wegel

5. Targeted Analysis of Chromatin Events (TACE)

Jason Sims, Changbin Chen, Peter Schlögelhofer, and Marie-Therese Kurzbauer

6. Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH)

Jason Sims, Jihed Chouaref, and Peter Schlögelhofer

Part II Cytological and Imaging Approaches to Study Meiosis in Other Species

7.  Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea

Zeeshan Shamim and Susan J. Armstrong

8. Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization

Stephen M. Stack, Lindsay A. Shearer, Leslie D. Lohmiller, and Lorinda K. Anderson

9. Cytological Techniques to Study Cytomixis in Plant Male Meiosis

Sergey R. Mursalimov, Yuriy V. Sidorchuk, and Elena V. Deineko

10. Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya Linn

José Mora-Calderón, Kalani Scott-Moraga, and Pablo Bolaños-Villegas

11. Analytical Methodology of Meiosis in Auto- and Allopolyploid Plants

Pilar Prieto and Tomás Naranjo

12. Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica Hybrids

Virginie Huteau and Olivier Coriton

13. Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy

Nieves Cuñado

14. Quantification of Synapsis using Immunolocalization in Embedded Nuclei of Lolium

Dylan W. Phillips

15. Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution Microscopy

Benoit Darrier, Mikel Arrieta, Sybille U. Mittmann, Pierre Sourdille, Luke Ramsay, Robbie Waugh, and Isabelle Colas

Part III Molecular and Biochemical Approaches to Study Meiosis

16. Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers

Christophe Lambing, Kyuha Choi, Alexander R. Blackwell, and Ian R. Henderson

17. Isolating Male Meiocytes from Maize and Wheat for “-omics” Analyses

Stefanie Dukowic-Schulze, Nelson Garcia, Arun S.K Shunmugam, Sateesh Kagale, and Changbin Chen

18. How to Study the Proteomes and Phosphoproteomes of Anther and Pollen

Chi Zhang, Xiaobing Fen, Menghui Hu, and Zaibao Zhang

19. Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development

Helian Liu, Aqin Cao, Liyu Yang, and Jianbo Wang

20. Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei

Steven Dreissig, Jörg Fuchs, Axel Himmelbach, Martin Mascher, and Andreas Houben

Part IV Further Approaches to Study Plants Meiosis and Beyond

21. Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops

Marina Pfalz, Adrián Gonzalo, Nicolas Christophorou, Aurélien Blary, Aurélie Berard, Nadia Bessoltane, Emilie Montes, Lydia Jaffrelo, Charles Poncet, Marie-Christine Le Paslier, Nathalie Nesi, Delphine Charif, and Eric Jenczewski

22. A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS)

Stuart Desjardins, Kostya Kanyuka, and James D. Higgins 

23. Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana

Nico De Storme and Danny Geelen

24. Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species

Dan D. Wu, Alevtina Ruban, Twan Rutten, Yong H. Zhou, and Andreas Houben

25. Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants

Annika Dorn and Holger Puchta

26. A Modular Tray Growth System for Barley

Mikel Arrieta, Isabelle Colas, Malcolm Macaulay, Robbie Waugh, and Luke Ramsay

27. In planta Delivery of Chemical Compounds into Barley Meiocytes – EdU as Compound Example

Yun-Jae Ahn, Maria Cuacos, Mohammad A. Ayoub, Judith Kappermann, Andreas Houben, and Stefan Heckmann

Blurb

This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field.