Plant Meiosis: Methods and Protocols

ISBN-13: 9781493959723 / Angielski / Miękka / 2016 / 238 str.

Wojtek P. Pawlowski; Mathilde Grelon; Susan Armstrong

Opis

This Methods in Molecular Biology (TM) book offers methods for molecular cytogenetics and chromosome analysis in plants. Includes state-of-the-art protocols for studying the organization and behavior of genetic material in a wide range of model and crop species.

Spis treści

Section I: CYTOLOGICAL TECHNIQUES FOR LIGHT MICROSCOPY

1. Spreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical Analysis

Susan Armstrong

 

2. Analysis of Plant Meiotic Chromosomes by Chromosome Painting

Martin A. Lysak and Terezie Mandáková

 

3. Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus

Elaine C. Howell and Susan Armstrong

 

4. Labeling Meiotic Chromosomes in Maize with Fluorescence in situ Hybridization

Zhi Gao, Fangpu Han, Tatiana V. Danilova, Jonathan C. Lamb, Patrice S. Albert, and James A.

Birchler

 

5. Examining Female Meiocytes of Maize by Confocal Microscopy

Philippa Barrell and Ueli Grossniklaus

 

6. Three-dimensional Acrylamide Fluorescence in situ Hybridization (FISH) for Plant Cells

Elizabeth S. Howe, Shaun P. Murphy, and Hank W. Bass

 

7. Analyzing Maize Meiotic Chromosomes with Super-resolution Structured Illumination Microscopy

Chung-Ju Rachel Wang

 

8. Live Imaging of Chromosome Dynamics

Moira J. Sheehan, R. Kelly Dawe, and Wojciech P. Pawlowski

 

9. Immunolocalization of Meiotic Proteins in Brassicacae – Method 1

Liudmila A. Chelysheva, Laurie Grandont, Mathilde Grelon

 

10. Immunolocalization of Meiotic Proteins in Arabidopsis thaliana - Method 2

Susan Armstrong and Kim Osman

 

11. Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana – Method 3

Xiaohui Yang, Li Yuan and Christopher A, Makaroff

 

12. A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana

Susan Armstrong

 

13. Analyzing Meiotic Chromosomes in Rice

Zhukuan Cheng

 

14. Analysing Meiosis in Barley

James D. Higgins

 

Section II: CYTOLOGICAL TECHNIQUES FOR ELECTRON MICROSCOPY

15. Preparing SC Spreads with RNs for EM Analysis

Lorinda K. Anderson and Stephen M. Stack

 

16. Analysis of the Synaptonemal Complex in Brassica using TEM

Susan Armstrong

 

17. Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy

Ljudmilla Timofejeva

 

 

Section III: GENETICS AND MOLECULAR BIOLOGY TECHNIQUES

18. Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen

Hossein Khademian, Laurène Giraut, Jan Drouaud, and Christine Mézard

 

19. Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize

Yan He, Gaganpreet Sidhu, and Wojciech P. Pawlowski

 

20. Analyzing the Meiotic Transcriptome using Isolated Meiocytes of Arabidopsis thaliana

Changbin Chen and Ernest F. Retzel

 

21. Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica using Affinity-based Proteomics

Kim Osman, Elisabeth Roitinger, Jianhua Yang, Susan Armstrong, Karl Mechtler, and F. Chris H. Franklin

 

22. Identifying Meiotic Mutants in Arabidopsis thaliana

Wayne Crismani and Raphaël Mercier

O autorze

Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These methods and protocols allows for understand the organization and behavior of the genetic material in a wide range of both model and crop species. extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.

Blurb

Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species.  Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.