Plant Lipid Signaling Protocols

ISBN-13: 9781493959273 / Angielski / Miękka / 2016 / 305 str.

Teun Munnik; Ingo Heilmann

Opis

As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.

Spis treści

Part I: Lipid Analyses

1. Analyzing Plant Signaling Phospholipids through ³²P_i-Labeling and TLC

Teun Munnik & Xavier Zarza

 2. Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC

Teun Munnik

 3. Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography

Mareike Heilmann and Ingo Heilmann

 4. Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay

Ingo Heilmann and Imara Y. Perera

 5. Quantification of Diacylglycerol by Mass Spectrometry

Katharina vom Dorp, Isabel Dombrink and Peter Dörmann

 6. Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK

Steven A. Arisz & Teun Munnik

 7. Use of Phospholipase A₂ for the Production of Lysophospholipids

Steven A. Arisz & Teun Munnik

 8. Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography

Vera Wewer, Peter Dörmann & Georg Hölzl

 9. Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry

Sunitha Shiva, Hieu Sy Vu, Mary R. Roth, Zhenguo Zhou, Shantan Reddy Marepally, Daya Sagar Nune, Gerald H. Lushington, Mahesh Visvanathan, and Ruth Welti

 10. Detection and Quantification of Plant Sphingolipids by LC-MS       

Jonathan E. Markham

 11. Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid

Nadja Stingl, Markus Krischke, Agnes Fekete  and Martin J. Mueller

 12. Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants
Sang-Chul Kim, Lionel Faure, and Kent D. Chapman

 13. Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State ³¹P NMR

Zachary T. Graber, and Edgar E. Kooijman

  Part II: Enzyme Activities

14. Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides

Sylvie Collin and Françoise Cochet

 15. Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays

Yang Ju Im, Irena Brglez, Catherine Dieck, Imara Perera and Wendy F. Boss

 16. Assaying Inositol and Phosphoinositide Phosphatase Enzymes

Janet L. Donahue, Mustafa Ercetin and Glenda E. Gillaspy

 17.  Determination of Phospholipase C Activity In Vitro

S.M. Teresa Hernández-Sotomayor and  J. Armando Muñoz-Sanchez

 18. Assaying Non-Specific Phospholipase C (NPC) Activity

Přemysl Pejchar, Günther F. E. Scherer and Jan Martinec

 19. Assaying Different Types of Plant Phospholipase D Activities In Vitro

Kirk L. Pappan and Xuemin Wang

 20. Measuring PLD Activity In Vivo

Teun Munnik & Ana M. Laxalt 

 21. Assaying Plant Phosphatidic Acid Phosphatase Activity

Yuki Nakamura

 22. Assay of Phospholipase A Activity

Michael Heinze and  Werner Roos

Jonathan E. Markham

 Part III: Protein-Lipid Intractions

23. Lipid-Binding Analysis using a Fat Blot Assay

Teun Munnik and Magdalena Wierzchowiecka

 24. Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions

Magdalena M. Julkowska, Johanna M. Rankenberg and Christa Testerink

 25. Lipid affinity beads – From Identifying New Lipid Binding Proteins to Assessing their Binding Properties

Fionn McLoughlin & Christa Testerink

 

Part IV:Imaging

26. Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants

Joop E.M. Vermeer and Teun Munnik

 27. Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy

Sarah J. Swanson and Simon Gilroy