Plant Gravitropism: Methods and Protocols

ISBN-13: 9781493955817 / Angielski / Miękka / 2016 / 323 str.

Elison B. Blancaflor

Opis

This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism.

Spis treści

1. Immunohistochemistry Relative to Gravity: A Simple Method to Retain Information About Gravity for Immunolocalization and Histochemistry 
Benjamin R. Harrison and Patrick H. Masson

2. A Flat Embedding Method to Orient Thin Biological Samples for Sectioning
Utku Avci and Jin Nakashima

3. Quantification of Root Gravitropic Response Using a Constant Stimulus Feedback System
Chris Wolverton

4. The Analysis of Gravitropic Setpoint Angle Control in Arabidopsis
Suruchi Roychoudhry and Stefan Kepinski

5. Imaging of Dynamic Ion Signaling During Root Gravitropism
Gabriele B. Monshausen

6. Live Cell Imaging of Cytoskeletal and Organelle Dynamics in Gravity-Sensing Cells in Plant Gravitropism
Moritaka Nakamura, Masatsugu Toyota, Masao Tasaka, and Miyo Terao Morita

7. Auxin Carrier and Signaling Dynamics During Gravitropic Root GrowthMugurel I. Feraru, Jürgen Kleine-Vehn, and Elena Feraru

8. Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements during Root Development and Gravitropism
Eveline Jacques, Darren Wells, Malcolm J. Bennett, and Kris Vissenberg

9. Methods for RNA Profiling of Gravi-Responding Plant Tissues
Jyoti Dalal, Eric Land, Naresh Vasani, Luyan He, Caroline Smith, Maria Rodriguez-Welsh, Imara Y. Perera, and Heike Sederoff

10. Proteomic Approaches and their Application to Plant Gravitropism
Proma Basu, Darron R. Luesse, and Sarah E. Wyatt

11. Assays for Root Hydrotropism and Response to Water Stress
Delfeena Eapen, Jesús J. Martínez, and Gladys I. Cassab

12. Evaluating Mechano-Transduction and Touch Responses in Plant Roots
Sarah J. Swanson, Richard Barker, Yonggeng Ye, and Simon Gilroy

13. Expressing and Characterizing Mechanosensitive Channels in Xenopus Oocytes
Grigory Maksaev and Elizabeth S. Haswell

14. Flowering Shoots of Ornamental Crops as a Model to Study Cellular and Molecular Aspects of Plant Gravitropism
Sonia Philosoph-Hadas, Haya Friedman, and Shimon Meir

15. Studying Molecular Changes During Gravity Perception and Response in a Single Cell
Ashley E. Cannon, Mari L. Salmi, Thomas J. Bushart, and Stanley J. Roux

16. Live Cell and Immuno-Labeling Techniques to Study Gravitational Effects on Single Plant Cells
Youssef Chebli and Anja Geitmann 

17. Use of High Gradient Magnetic Fields to Evaluate Gravity Perception and Response Mechanisms in Plants and Algae
Karl H. Hasenstein

18. Use of Microgravity Simulators for Plant Biological Studies
Raúl Herranz, Miguel A. Valbuena, Aránzazu Manzano, Khaled Youssef, and F. Javier Medina

19. Conducting Plant Experiments in Space
John Z. Kiss

20. Spaceflight Exploration in Plant Gravitational Biology
Anna-Lisa Paul and Robert J. Ferl

21. Hypergravity Experiments to Evaluate Gravity Resistance Mechanisms in Plants
Kouichi Soga, Sachiko Yano, Shouhei Matsumoto, and Takayuki Hoson

O autorze

Elison B. Blancaflor Professor Plant Biology Division 2510 Sam Noble Parkway The Samuel Roberts Noble Foundation Ardmore, OK 73401 Office phone: 580-224-6687 Fax: 580-224-6692 eblancaflor@noble.org

Blurb

This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data collection and image analysis; and techniques to investigate ion, organelle, and auxin transporter dynamics, particularly in living cells, as the plant is responding to a change in its orientation. The next few chapters talk about topics that are essential for understanding the complexities underlying tropisms and plant movements in general, and outline basic protocols on handling ornamental flowering shoots for basic plant gravitropism studies. The book concludes with chapters that discuss plant biological studies in space in order to take advantage of unique microgravity conditions not available in Earth-based studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Plant Gravitropism is the perfect book for researchers in the plant scientific community because it is not only useful for plant gravitropism studies, but also addresses a range of interesting problems in plant growth and development.