Plant Gravitropism: Methods and Protocols

ISBN-13: 9781071616796 / Angielski / Miękka / 2022 / 323 str.

Elison B. Blancaflor

Opis

This updated and expanded edition explores key methodologies to study the fascinating phenomenon of how plants readjust their growth toward gravity. In addition to the protocols delivering broad applications for gaining insight into other plant physiological processes, this new volume also focuses on techniques involving plants in space or the use of microgravity analogs to study plant biological phenomenon. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Plant Gravitropism: Methods and Protocols, Second Edition serves as an ideal guide for researchers studying the cellular, molecular, and biochemical networks that plants use to translate environmental stimuli into a growth response.

Spis treści

1. Plant Gravitropism: From Mechanistic Insights into Plant Function on Earth to Plants Colonizing Other Worlds

            Sabrina Chin and Elison B. Blancaflor

 

2. Evaluation of Gravitropism in Non-Seed Plants

            Yuzhou Zhang, Lanxin Li, and Jiří Friml

 

3. Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii

            Ashley E. Cannon, Tanya Sabharwal, and Stanley J. Roux

 

4. A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates

            Takehiko Ogura, Christian Goeschl, and Wolfgang Busch

 

5. Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms

            Jinke Chang and Jia Li

 

6. Using the Automated Botanical Contact Device (ABCD) to Deliver Reproducible, Intermittent Touch Stimulation to Plants

            Caleb Fitzgerald, Cullen S. Vens, Nathan Miller, Richard Barker, Matthew Westphall, Johnathan Lombardino, Jerry Miao, Sarah J. Swanson, and Simon Gilroy

 

7. High-Resolution Kinematic Analysis of Root Gravitropic Bending Using RootPlot

            Aditi Bhat, Cody L. DePew, and Gabriele B. Monshausen

 

8. Staging of Emerged Lateral Roots in Arabidopsis thaliana

            Sascha Waidmann and Jürgen Kleine-Vehn

 

9. Methods for a Quantitative Comparison of Gravitropism and Posture Control Over a Wide Range of Herbaceous and Woody Species

            Félix P. Hartmann, Hugo Chauvet-Thiry, Jérôme Franchel, Stéphane Ploquin, Bruno Moulia, Nathalie Leblanc-Fournier, and Mélanie Decourteix

 

10. The Analysis of Gravitropic Setpoint Angle Control in Plants

            Suruchi Roychoudhry, Marta Del Bianco, and Stefan Kepinski

 

11. A Flat Embedding Method to Orient Gravistimulated Root Samples for Sectioning

            Utku Avci and Jin Nakashima

 

12. Conducting Plant Experiments in Space and on the Moon

            Tatsiana Shymanovich and John Z. Kiss

 

13. Plant Proteomic Data Acquisition and Data Analyses: Lessons from Spaceflight

            Proma Basu, Colin P.S. Kruse, Darron R. Luesse, and Sarah E. Wyatt

 

14. Blueprints for Constructing Microgravity Analogs

            Karl H. Hasenstein

 

15. Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies

            Alexander Meyers, Nathan Scinto-Madonich, Sarah Wyatt, and Chris Wolverton

 

16. Use of Reduced Gravity Simulators for Plant Biological Studies

            Raúl Herranz, Miguel A. Valbuena, Aránzazu Manzano, Khaled Y. Kamal, Alicia Villacampa, Malgorzata Ciska, Jack J.W.A. van Loon, and F. Javier Medina

 

17. Understanding the Mechanisms of Gravity Resistance in Plants

            Kouichi Soga, Sachiko Yano, Motoshi Kamada, Shouhei Matsumoto, and Takayuki Hoson

 

18. NASA’s Ground-Based Microgravity Simulation Facility

            Ye Zhang, Jeffery T. Richards, Jessica L. Hellein, Christina M. Johnson, Julia Woodall, Tait Sorenson, Srujana Neelam, Anna Maria J. Ruby, and Howard G. Levine

 

19. Evaluating the Effects of the Circadian Clock and Time of Day on Plant Gravitropic Responses

            Joseph S. Tolsma, Jacob J. Torres, Jeffrey T. Richards, Imara Y. Perera, and Colleen J. Doherty