Plant Gene Silencing: Methods and Protocols

ISBN-13: 9781071618745 / Angielski / Twarda / 2022

Kirankumar Mysore; Muthappa Senthil-Kumar

Spis treści

1. Recent Advances in Plant Gene Silencing Methods

Prachi Pandey, Kirankumar S. Mysore^, and Muthappa Senthil-Kumar

 

2. Strategies for Efficient RNAi Based Gene Silencing of Viral Genes For Disease Resistance In Plants 

Krish K Kumar, Shanmugam Varanavasiappan, Loganathan Arul, Easwaran Kokiladevi, and Duraialagaraja Sudhakar

 

3. Genome Editing and Designer Crops for the Future

Sumi Rana, Pooja Rani Aggarwal, Varsa Shukla, Urmi Giri, Shubham Verma, and Mehanathan Muthamilarasan

 

4. In Silico Methods for the identification of Viral-derived Small interfering RNAs (vsiRNAs) and their application in Plant genomics

Aditya Narayan, Shafaque Zahra, Ajeet Singh, and Shailesh Kumar

 

5. Barley stripe mosaic virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes In Wheat And Barley

Harvinder Bennypaul and Upinder S. Gill

 

6. Virus-induced Gene Silencing in Wheat and Related Monocot Species

Vinay Panwar and Kostya Kanyuka

 

7. Virus-Induced Gene Silencing in Sorghum using Brome mosaic virus

Dharmendra K. Singh and Kirankumar S. Mysore

 

8. RTBV-based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications

Gaurav Kumar, Kamlesh Kumari, and Indranil Dasgupta

 

9. Optimization of Tobacco rattle virus (TRV)-based Virus-Induced Gene Silencing (VIGS) in Tomato

Ashish Kumar Singh, Dibyendu Ghosh, and Supriya Chakraborty

 

10. Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal  Plants

     Dikki Pedenla Bomzan, Krishna Kumar, Sarma Rajeev Kumar, Seema Meena, and         Dinesh A. Nagegowda

 

11. VIGS-based Gene Silencing for Assessing Mineral Nutrient Acquisition

Akash, Rajat Srivastava, and Rahul Kumar

 

12. High throughput Analysis of Gene Function Under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants

Ramegowda Yamunarani, Venkategowda Ramegowda, Muthappa Senthil‐Kumar, and Kirankumar S Mysore

13. A Method for Developing RNAi-derived Resistance in Cowpea Against Geminiviruses

Sanjeev Kumar, Sunil Kumar Mukherjee, Lingaraj Sahoo

 

14. In vitro method for synthesis of large scale ds RNA molecule as a Novel Plant Protection Strategy

Sundaresha, S, Aarti Bairava, Maharishi Tomar, Ravinder Kumar, E.P. Venkatasalam, Vinay Sagar, Vinay Bhardwaj, and Sanjeev Sharma

 

15. Fine-tuning Plant Gene Expression with Synthetic Trans-Acting Small Interfering RNAs

Lucio López-Dolz, Maria Spada, José-Antonio Daròs, and Alberto Carbonell

 

16. Trans-Kingdom RNA silencing in Plant-Fungal Disease Control

Tao Zhang, Fei Wang, Hui-Shan Guoand Yun Jin

 

17. An integrated bioinformatics and functional approach for miRNA validation

Sombir Rao, Sonia Balyan, Chandni Bansal, and Saloni Mathur

 

18. Development of a Ligation-Independent Cloning-Based Dual Vector System for RNA Interference in Plants

Jinping Zhao, Carlos Garcia Rios, Jingjing Xu, Ijaz Ahmad, and Junqi Song

 

19. Multigene Transformation Through Cre-lox mediated Site-Specific Integration in Rice

Bhuvan Pathak, Soumen Nandy, and Vibha Srivastava

 

20. An Improvised Hairy Root Transformation Method for Efficient Gene Silencing in Roots and Nodules of Arachis hypogaea

Bikash Raul and Senjuti Sinharoy

 

21. A method to reduce off-targets in CRISPR/Cas9 system in plants

Ali Movahedi, Zahra Hajiahmadi, Hui Wei, Liming Yang, Honghua Ruan, and Qiang Zhuge

Blurb

This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.