Plant Fungal Pathogens: Methods and Protocols

ISBN-13: 9781493958986 / Angielski / Miękka / 2016 / 648 str.

Melvin D. Bolton; Bart P. H. J. Thomma

Opis

Including reproducible laboratory protocols, this guide to fungal pathogens in plants has been written by expert researchers in the field and includes methods now used to study them, including techniques for model systems such as Arabidopsis thaliana.

Recenzje

From the reviews:

"The authors provide the latest information on topics including methods in molecular biology and highlighting some of the latest techniques critical for students pursuing molecular plant pathology. ... All the chapters are well illustrated with charts and graphs and with necessary photographs. ... should be available in school libraries experienced fungal research laboratories and university libraries. This is specially aimed at researchers new to the molecular biology field." (Asha Janadaree Dissanayake and Kevin D. Hyde, Fungal Diversity, Vol. 59, March, 2013)

"The current volume is a unique collection of 41 chapters from a diverse group of scientists, researchers, and academics working on different aspects of the molecular basis of fungal pathogens and pathogenesis. ... This volume will be useful for both students and researchers in the fields of botany and forestry, agriculture, mycology, microbiology, plant disease and diagnostics, molecular plant pathology, horticulture science, biomolecular sciences, life sciences, fungal molecular biology, fungal biotechnology, microbial biotechnology, and food technology." (S. K. Basu, Plant Science Bulletin, Vol. 59 (1), 2013)

Spis treści

1.  Fungal ABC Transporter Deletion and Localization AnalysisAndriy Kovalchuk, Stefan Weber, Jeroen G. Nijland, Roel A.L. Bovenberg, and Arnold J.M. Driessen2. Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated Transformation

Rasmus John Normand Frandsen, Mette Frandsen, Henriette Giese

3. Activity-based Protein Profiling of Infected Plants

Farnusch Kaschani, Christian Gu, Renier A.L. van der Hoorn

4. The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves

Lisong Ma, Ewa Lukasik, Fleur Gawehns and Frank L.W. Takken

5. Proteomic Techniques for Plant-Funal Interactions

Delphine Vincent, Kar-Chun Tan, Liam Cassidy, Peter Solomon and Richard Oliver

6. Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway^®-Compatible Binary Potato Virus X-Expression Vector

H. Peter van Esse

7. Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens 

Jayma A. Moore and Scott A. Payne

8. Real-time PCR for the Quantification of Fungi in planta

Steven J. Klosterman

9. Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi

Mark Arentshorst, Arthur F.J. Ram, Vera Meyer

10. Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates

Elizabeth Adams,Danielle Emerson, Sean Croker, Hye-Seon Kim^, Shannon Modla, Seogchan Kang, Kirk Czymmek

11. Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors

Shunwen Lu

12. Characterization of Plant – Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens

Timothy L. Friesen and Justin D. Faris

 13.Heterologous Production of Fungal Effectors in Pichia pastoris

Anja Kombrink

 14. The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta

Wei-Hua Tang, Yan Zhang, and Jon Duvick

15. Metabolomics Protocols for Filamentous Fungi

Joel P.A. Gummer, Christian Krill, Lauren Du Fall, Ormonde D.C. Waters^, Robert D. Trengove, Richard P. Oliver and Peter S. Solomon

16. Targeted Gene Replacement in Fungi using a Split-Marker Approach

Rubella S. Goswami

17.Massively Parallel Sequencing Technology in Pathogenic Microbes

Sucheta Tripathy and Rays H.Y. Jiang

 18.Confocal Microscopy in Plant Pathogen Interactions

Adrienne R. Hardham

 19.The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data

Benjamin M. Nitsche, Arthur F.J. Ram, Vera Meyer

 20.Population Biology of Fungal Plant Pathogens

Zahi K. Atallah and Krishna V. Subbarao

 21.Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens

Zhaohui Liu and Timothy L. Friesen

  22.In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi

W.R. Rittenour, S.D. Harris

 23.Fungicide Resistance Assays for Fungal Plant Pathogens

Gary A. Secor and Viviana V. Rivera

 24.Identification of Lipid-binding Effectors

Shiv D. Kale and Brett M. Tyler

25. In silico Identification and Characterization of Effector Catalogs

Ronnie de Jonge

 26. Horizontal Transfer of Supernumerary Chromosomes in Fungi

H. Charlotte van der Does and Martijn Rep

 27. The Induction of Mycotoxins by Trichothecene Producing Fusarium Species

Rohan Lowe, Mélanie Jubault, Gail Canning, Martin Urban and Kim E. Hammond-Kosack

 28. Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics

J.B. Ridenour, R.L. Hirsch, and B.H. Bluhm

 29.Assessment of Autophagosome Formation by Transmission Electron Microscopy (TEM)

Marina Nadal and Scott E. Gold

^30. Fungal Plant Pathogen Detection in Plant and Soil Samples using DNA Macroarrays

B Lievens, A Justé and K A Willems

 

31. Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors

Parthasarathy Santhanam

 

32. A Yeast-Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and their Plant Hosts

Sang-Jik Lee and Jocelyn K.C. Rose

 33. Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes

Eva H. Stukenbrock and Julien Y. Dutheil

 34.Multi-gene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens

Tara L. Rintoul , Quinn A. Eggertson , and C. André Lévesque

 35.MAP Kinase Phosphorylation and cAMP Assessment in Fungi

Rahim Mehrabi, Sarrah Ben M’Barek, Abbas Saidi, Masoud Abrinbana, Mostafa Aghaee, Pierre J. G. M. de Wit and Gert H. J. Kema

 36.A One-step Affinity-purification Protocol to Purify NB-LRR Immune Receptors from Plants that Mediate Resistance to Fungal Pathogens

Wladimir I.L. Tameling

 37.Karyotyping Methods for Fungi

Rahim Mehrabi, Masatoki Taga, Mostafa Aghaee, Pierre J. G. M. de Wit, Gert H. J. Kema

 38.Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique

Matthieu H.A.J. Joosten

 39.Gene Cloning using Degenerate Primers and Genome Walking

Javier A. Delgado, Steven Meinhardt, Samuel G. Markell and Rubella S. Goswami

 40.Construction of Hairpin RNA Expressing Vectors for RNA-mediated Gene Silencing in Fungi

Shaobin Zhong, Yueqiang Leng, Melvin D. Bolton

  41.An Unbiased Method for the Quantitation of Disease Phenotypes using a Custom-built Macro Plugin for the Program ImageJ

Ahmed Abd-El-Haliem

Blurb

Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor.  In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.