Plant Epigenetics: Methods and Protocols

ISBN-13: 9781493979561 / Angielski / Miękka / 2018 / 253 str.

Igor Kovalchuk

Spis treści

1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis

Wanhui You, Stéphane Pien, and Ueli Grossniklaus

 

2. Chromatin Conformation Capture-Based Analysis of Nuclear Architecture

Stefan Grob and Ueli Grossniklaus

 

3. Metaanalysis of Genome-Wide Chromatin Data

Julia Engelhorn and Franziska Turck

 

4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes

Sara Hernández-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peña

 

5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns

Andriy Bilichak and Igor Kovalchuk

 

6. Analysis of Global Genome Methylation Using the Cytosine-Extension Assay

Andriy Bilichak and Igor Kovalchuk

 

7. In Situ Analysis Of DNA Methylation in Plants

Palak Kathiria and Igor Kovalchuk

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8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay

Andrey Golubov and Igor Kovalchuk

 

9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)

María Ángeles Guevara, Nuria De María, Enrique Sáez-Laguna, María Dolores Vélez, María Teresa Cervera, and José Antonio Cabezas

 

10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes

Pavlina Sasheva Ueli Grossniklaus

 

11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays

Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot

 

12. Northern Blotting Techniques for Small RNAs

Todd Blevins

 

13. Stem-Loop QRT-PCR for the Detection of Plant Micrornas

Erika Varkonyi-Gasic

 

14. Profiling New Small RNA Sequences

Masayuki Tsuzuki and Yuichiro Watanabe

 

15. Small RNA Library Preparation and Illumina Sequencing in Plants

Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk

 

16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow<

Slava Ilnytskyy and Andriy Bilichak

 

17. Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing

Andriy Bilichak and Igor Kovalchuk

 

18. The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks

Andriy Bilichak and Igor Kovalchuk

 

19. Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species

Christian Parisod