Plant Embryogenesis: Methods and Protocols

ISBN-13: 9781071603413 / Angielski / Twarda / 2020 / 298 str.

Martin Bayer

Spis treści

Part I: Mutagenesis and Genetic Screens

1. Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize

C. Stewart Gillmor, A. Mark Settles, and Wolfgang Lukowitz

 

2. EMS Mutagenesis of Arabidopsis Seeds

C. Stewart Gillmor and Wolfgang Lukowitz

 

3. EMS Mutagenesis of Maize Pollen

A. Mark Settles

 

Part II: Microscopic Imaging and Phenotypic Analysis of Arabidopsis Embryos

4. Live-cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana

Minako Ueda, Yusuke Kimata, and Daisuke Kurihara

 

5. Analyzing Sub-Cellular Reorganization during Early Arabidopsis Embryogenesis using Fluorescent Markers

Che-Yang Liao and Dolf Weijers

 

6. From Stained Plant Tissues to Quantitative Cell Segmentation Analysis with MorphoGraphX

Merijn Kerstens, Soeren Strauss, Richard Smith, and Viola Willemsen

 

Part III: Expression Analysis and Transcriptomics

7. Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos

Katalin Páldi, Magda Mosiolek, and Michael D. Nodine

 

8. Manual Isolation of Living Early Embryos from Tobacco Seeds

Peng Zhao, Xuemei Zhou, Ce Shi, and Meng-xiang Sun

 

9. Profiling Transcriptomes of Manually Dissected Arabidopsis Embryos

Ping Kao and Michael D. Nodine

 

10. Laser-assisted Microdissection of Plant Embryos for Transcriptional Profiling

Ana Marcela Florez-Rueda, Lucas Waser, and Ueli Grossniklaus

 

11. Cell-type-specific Transcriptomics in the Plant Embryo Using an Adapted INTACT Protocol

Joakim Palovaara and Dolf Weijers

 

12. Transcriptomic Profiling of the Arabidopsis Embryonic Epidermis Using FANS in Combination with RNAseq

Daniel Slane, Kenneth W. Berendzen, Janika Witthöft, and Gerd Jürgens

 

Part IV: Protein Interactions Studies

13. Visualizing Protein Associations in living Arabidopsis Embryo

Yuchen Long,Yvonne Stahl,Stefanie Weidtkamp-Peters, and Ikram Blilou

 

Part V: Embryogenesis in Other Model and Non-Model Species

14. Imaging of Embryo Sac and Early Seed Development in Maize after Feulgen Staining

Kamila Kalinowska, Junyi Chen, and Thomas Dresselhaus

 

15. Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis

Min Chen, Anhthu Q. Bui, and Robert B. Goldberg

 

16. Microscopical Detection of Cell Death Processes during Scots Pine Zygotic Embryogenesis

Jaana Vuosku and Suvi Sutela

 

Part VI: In Vitro Systems to Study Embryogenesis

17. Regulation of Somatic Embryo Development in Norway Spruce

Sara von Arnold, Tianqing Zhu, Emma Larsson, Daniel Uddenberg, and David Clapham

 

18. In vitro Production of Zygotes by Electro-Fusion of Rice Gametes

Md Hassanur Rahman, Erika Toda, and Takashi Okamoto

 

19. Isolated Microspore Culture in Brassica napus

Patricia Corral-Martínez, Carolina Camacho-Fernández, and Jose M. Seguí-Simarro

 

20. Anther Culture in Eggplant (Solanum melongena L.)

Antonio Calabuig-Serna, Rosa Porcel, Patricia Corral-Martínez, and Jose M. Seguí-Simarro

Blurb

This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

The chapter “Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.