Plant Embryogenesis

ISBN-13: 9781617378485 / Angielski / Miękka / 2010 / 184 str.

Maria Fernanda Suarez; Peter V. Bozhkov

Opis

• Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips.
• Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism.
• Provides the most comprehensive collection of protocols on plan embryo development.

Recenzje

From the reviews:

"I wish to recommend ... 'Plant Embryogenesis' edited by M.F. Suárez and P.V. Bozhkov. ... we can whole-heartedly express our thanks to all authors - leading experts in the field of plant embryology for very useful book. ... The authors aim this book primarily at researchers who are relatively new to the field of plant embryology. ... this volume will be very useful text in all laboratories dealing with plant development and embryology. It can inspire students as well as full-fledged plant embryologists." (Z. Vondráková, Biologia Plantarum, Vol. 52 (2), 2008)

Spis treści

Contents PART I. MODEL EMBRYONIC SYSTEMS 1. Arabidopsis embryogenesis Soomin Park and John J. Harada 2. Maize embryogenesis Pilar Fontanet and Carlos M. Vicient 3. Spruce embryogenesis Sara von Arnold and David Clapham PART II. CELLULAR, GENETIC AND MOLECULAR MECHANISMS OF PLANT EMBRYOGENESIS 4. In vitro fertilization with isolated higher plant gametes Erhard Kranz, Yoichiro Hoshino and Takashi Okamoto 5. In vitro culture of Arabidopsis embryos Michael Sauer and Jirí Friml 6. Culture and time-lapse tracking of barley microspore-derived embryos Simone de F. Maraschin, Sandra van Bergen, Marco Vennik and Mei Wang 7. Isolation of embryo-specific mutants in Arabidopsis: Plant transformation Nai-You Liu, Zhi-Feng Zhang and Wei-Cai Yang 8. Isolation of embryo-specific mutants in Arabidopsis: Genetic and phenotypic analysis Nai-You Liu, Zhi-Feng Zhang and Wei-Cai Yang 9. Laser-capture microdissection to study global transcriptional changes during plant embryogenesis Stuart A. Casson, Matthew W.B., Spencer and Keith Lyndsey 10. Promoter trapping system to study embryogenesis Robert Blanvillain and Patrick Gallois 11. Visualization of auxin gradients in embryogenesis Michael Sauer and Jirí Friml 12. Intercellular trafficking of macromolecules during embryogenesis Insoon Kim and Patricia C. Zambryski 13. Immunolocalisation of proteins in somatic embryos: applications for studies on the cytoskeleton Andrei P. Smertenko and Patrick J. Hussey 14. Detection of programmed cell death in plant embryos Lada H. Filonova, Maria F. Suarez and Peter V. Bozhkov

Blurb

The advent of the postgenome era has brought a remarkable evolution in modern plant embryology. In Plant Embryogenesis, the authors focus on the common developmental process and compile novel tools and methods designed specifically for studying plant embryogenesis. In each chapter, detailed outlines of model embryonic systems are provided, as well as cutting-edge cellular, genetic and molecular mechanisms. This important book gives insight into the methods used in studying the transformation of phylogenetically-conserved embryonic patterns into flora’s astounding diversity of growth forms today. Invaluable to those new to the field and inspiring to full-fledged plant embryologists, Plant Embryogenesis provides today’s most comprehensive platform to the understanding of plant embryo development.