Plant Embryo Culture: Methods and Protocols

ISBN-13: 9781617379871 / Angielski / Twarda / 2011 / 377 str.

Trevor A. Thorpe; Edward C. Yeung

Opis

A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book's main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.

Spis treści

Part I: Culture of Zygotic Embryos: Fundamental Studies

 

1. Zygotic Embryo Culture: An Overview

            Tegan M. Haslam and Edward C. Yeung

 

2. In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture

            Takashi Okamoto

 

3. Canola Zygotic Embryo Culture

            Nicole S. Ramesar-Fortner and Edward C. Yeung

 

4. Immature Seeds and Embryos of Medicago truncatula Cultured In vitro

            Sergio J. Ochatt

 

5. In vitro Culture and Germination of Terrestrial Asian Orchid Seeds

            Yung-I Lee

 

6. In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos

            Florent Engelmann, Bernard Malaurie, and Oulo N’Nan

 

Part II: Application of Embryo Culture Techniques: Embryo Rescue

 

7. Immature Embryo Rescue and Culture

            Xiuli Shen, Fred G. Gmitter, Jr., and Jude W. Grosser

 

8. Chickpea Hybridization Using In vitro Techniques

            Nalini Mallikarjuna and Fred J. Muehlbauer

 

9. Muskmelon Embryo Rescue Techniques using In vitro Embryo Culture

            Hector Gordon Nuñez-Palenius, Rafael Ramírez-Malagón, and Neftalí Ochoa-Alejo

 

10. Phaseolus Immature Embryo Rescue Technology

            Pascal Geerts, André Toussaint, Guy Mergeai, and Jean-Pierre Baudoin

 

11. Wide Crossing in Lentil through Embryo Rescue

            Richard Fratini and Maria L. Ruiz

 

12. Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques

            Ajoy Kumar Roy, Devendra Ram Malaviya, and Pankaj Kaushal

 

Part III: Cryopreservation of Embryos

 

13. Cryopreservation of Embryos: An Overview

            Florent Engelmann

 

14. Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm

            Anna De Carlo, Maurizio Lambardi, and Elif Aylin Ozudogru

 

15. Cryopreservation of Zygotic Embryo Axes and Somatic Embryos of European Chestnut

            Ana M. Vieitez, M. Carmen San-José, and Elena Corredoira

 

16. Cryopreservation of Ilex Immature Zygotic Embryos

            Luis Mroginski, Natalia Dolce, Pedro Sansberro, Claudia Luna, Ana Gonzalez, and Hebe Rey

 

Part IV: The Use of Zygotic Embryos as Explants for Somatic Embryogenesis and/or Organogenesis

 

17. The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview

            Mohamed Elhiti and Claudio Stasolla

 

18. Somatic Embryogenesis and Plant Regeneration in the Culture of Arabidopsis thaliana (L.) Heynh. Immature Zygotic Embryos

            Malgorzata D. Gaj

 

19. Pine Somatic Embryogenesis Using Zygotic Embryos as Explants

            Gerald S. Pullman and Kylie Bucalo

 

20. Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies

            Kee Yoeup Paek, Eun Joo Hahn, and So Young Park

 

Part V: Transformation Using Zygotic Embryos as Explants

 

21. Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview

            Muhammad Tahir, Ejaz A. Waraich, and Claudio Stasolla

 

22. Genetic Transformation Using Maize Immature Zygotic Embryos

            Bronwyn Frame, Marcy Main, Rosemarie Schick, and Kan Wang

 

23. Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue

            Martha M. O’Kennedy, Hester C. Stark, and Nosisa Dube

 

24. Agrobacterium tumefaciens-Mediated Genetic Transformation of Cereals Using Immature Embryos

            Ashok K. Shrawat and Allen G. Good