Plant Circular Rnas: Methods and Protocols

ISBN-13: 9781071616444 / Angielski / Twarda / 2021 / 196 str.

Luis Maria Vaschetto

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Identification and Characterization of Plant Circular RNAs
Yan-Fei Zhou, Yu-Meng Sun, and Yue-Qin Chen

2. Experimental Strategies for Studying the Function of Plant circRNAs
Yan-Zhao Feng and Yang Yu

3. Generation of Transgenic Rice Expressing circRNA and its Functional Characterization
Priyanka Sharma, Ashirbad Guria, Sankar Natesan, and Gopal Pandi

4. Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development
Ashirbad Guria, Priyanka Sharma, Sankar Natesan, and Gopal Pandi

5. Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing 
Taiyun Wang, Xiaotuo Zhang, and Binglian Zheng

6. Identification and Functional Characterization of Viroid Circular RNAs 
José-Antonio Daròs

7. Circular RNA Databases
Peijing Zhang and Ming Chen

8. NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs
Laura Carmen Terrón-Camero and Eduardo Andrés-León

9. Computational Analysis of Transposable Elements and circRNAs in Plants
Liliane Santana Oliveira, Andressa Caroline Patera, Douglas Silva Domingues, Danilo Sipoli Sanches, Fabricio Martins Lopes, Pedro Henrique Bugatti, Priscila Tiemi Maeda Saito, Vinicius Maracaja-Coutinho, Alan Mitchell Durham, and Alexandre Rossi Paschoal

10. Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database
Jinjing Zhang, Ruiqi Liu, and Guanglin Li

11. Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases
Nureyev F. Rodrigues and Rogerio Margis

Subject Index List…

Blurb

This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs’ research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field.