Plant Chemical Genomics: Methods and Protocols

ISBN-13: 9781493959518 / Angielski / Miękka / 2016 / 269 str.

Glenn R. Hicks; Stephanie Robert

Opis

Plant Chemical Genomics: Methods and Protocols , expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall. The presentation of these and other examples as well as synthetic chemistry, computation and target identification provides a comprehensive overview of the practical aspects of chemical biology that are possible in plant systems. Small molecules and natural products that produce a phenotype in plants have been known for decades. The agrichemical industry has utilized small molecules based on known natural molecules such as auxins, cytokinins, abscissic acid and other growth regulators. In recent years, the screening and characterization of novel small molecules has enhanced our understanding of protein function, metabolic, signaling and endomembrane pathways, and their interactions in basic plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Spis treści

Section I: Automation and Imaging

1. Fully-automated Compound Screening in Arabidopsis thaliana Seedlings

Dominique Audenaert, Long Nguyen, Bert De Rybel, and Tom Beeckman

 

2. Time-profiling Fluorescent Reporters in the Arabidopsis Root

Antoine P. Larrieu, Andrew P. French, Tony P. Pridmore, Malcolm J. Bennett and Darren M. Wells

 

3. Screening for Bioactive Small Molecules by in vivo Monitoring of Luciferase-based Reporter Gene Expression in Arabidopsis thaliana

Christian Meesters and Erich Kombrink

 

 

Section II: Chemical Screening

4. Application of Yeast-two Hybrid Assay to Chemical Genomic Screens: A High-throughput System to Identify Novel Molecules Modulating Plant Hormone Receptor Complexes

Andrea Chini

 

5. High-throughput Screening of Small Molecule Libraries for Inducers of Plant Defense Responses

Colleen Knoth and Thomas Eulgem

 

6. Using a Reverse Genetics Approach to Investigate Small Molecule Activity

Siamsa M. Doyle and  Stéphanie Robert

 

7. Investigating the Phytohormone Ethylene Response Pathway by Chemical Genetics

Lee-Chung, Lin, Chiao-Mei Chueh, and Long-Chi Wang

 

8. Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in membrano and in Planta

Laurence Boudière and Eric Maréchal

 

9. Forward Chemical Screening of Small RNA Pathways

Yifan Lii and Hailing Jin

 

10. Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening

Charles T. Anderson and Andrew Carroll

 

11. High-throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen

Michelle Q. Brown, Nolan Ung, Natasha V. Raikhel, and Glenn R. Hicks

 

12. Plant Chemical Genomics: Gravity Sensing and Response

Marci Surpin

 13. Screening Chemical Libraries for Compounds that Affect Protein Sorting to the Yeast Vacuole

Jan Zouhar

 

Section III: Cheminformatics

14. The use of Multi-drug Approach to Uncover New Players of the Endomembrane System Trafficking Machinery

Urbina D, Pérez-Henríquez P,  and Norambuena L

 15. Cheminformatic Analysis of High-Throughput Compound Screens

Tyler W. H. Backman and Thomas Girke

 

16. Endomembrane Dissection Using Chemically-Induced Bioactive Clusters

Natasha Worden, Thomas Girke, and Georgia Drakakaki

 17. Statistical Molecular Design: A Tool to Follow Up Hits From Small Molecule ScreeningAnders E. G. Lindgren, Andreas Larsson, Anna Linusson, and Mikael Elofsson

 

 

Section IV: Target Identification

18. Early Stage Hit Triage for Plant Chemical Genetic Screens and Target Site Identification

Terence A. Walsh

 

19. Screening for Gene Function using the FOX (Full-length cDNA OvereXpressor gene) Hunting System

Mieko Higuchi, Minami Matsui

 

 

 

Section V: Hormone Transport and Metabolite Profiling

20. Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry

Jan Huege, Jan Goetze, Frederik Dethloff, Bjoern Junker, and Joachim Kopka

 

21. ¹H NMR Based Metabolomics Methods for Chemical Genomics Experiments

Daniel J. Orr, Gregory A. Barding, Jr., Christiana E. Merrywell, Glenn R. Hicks, Natasha V. Raikhel, and Cynthia K. Larive

 

22. Determination of Auxin Transport Parameters on the Cellular Level

Jan Petrášek, Martina Laňková, and Eva Zažímalová

 

23. Analyzing the in vivo Status of Exogenously Applied Auxins: A HPLC-based Method to Characterize the Intracellularly Localized Auxin Transporters

Sibu Simon, Petr Skůpa, Petre I. Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml