Plant Cell Morphogenesis: Methods and Protocols

ISBN-13: 9781493994717 / Angielski / Miękka / 2020 / 380 str.

Fatima Cvrčková;Viktor Zarsky

Opis

This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs.

Spis treści

1. Essential Methods of Plant Sample Preparation for Light Microscopy

            Aleš Soukup and Edita Tylová

 

2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy

            Aleš Soukup

 

3. Chemical Fixation, Immunofluorescence, and Immunogold Labelling of Electron Microscopical Sections

            Ilse Foissner and Margit Hoeftberger

 

4. Essential Methods of Plant Sample Preparation for High Resolution Scanning Electron Microscopy at Room Temperature

            Jana Nebesářová

 

5. Fluorescence Lifetime Imaging of Plant Cell Walls

            Christine Terryn and Gabriel Paës

 

6. Raman Spectroscopy in Non-Woody Plants

            Dorota Borowska-Wykręt and Mateusz Dulski

 

7. Image Analysis: Basic Procedures for Description of Plant Structures

            Jana Albrechtová, Zuzana Kubínová, Aleš Soukup, and Jiří Janáček

 

8. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing

            Fatima Cvrčková

 

9. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics

            Amparo Rosero, Denisa Oulehlová, Viktor Žárský, and Fatima Cvrčková

 

10. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D

            Birgit Möller, Luise Zergiebel, and Katharina Bürstenbinder

 

11. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions

            Adriana Jelínková, Kateřina Malínská, and Jan Petrášek

 

12. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells

            Lise C. Noack, Přemysl Pejchar, Juraj Sekereš, Yvon Jaillais, and Martin Potocký

 

13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics

            Alexandra Lešková, Zuzana Kusá, Mária Labajová, Miroslav Krausko, and Ján Jásik

 

14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells

            Mateusz Majda, Aleksandra Sapala, Anne-Lise Routier-Kierzkowska, and Richard S. Smith

 

15. Optical Trapping in Plant Cells

            Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren

 

16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation

            Dorota Kwiatkowska, Sandra Natonik-Białoń, and Agata Burian

 

17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope

            Olivier Hamant, Pradeep Das, and Agata Burian

 

18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX

            Soeren Strauss, Aleksandra Sapala, Daniel Kierzkowski, and Richard S. Smith

 

19. Kinematic Characterization of Root Growth by Means of Stripflow

            Tobias I. Baskin and Ellen Zelinsky

 

20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens

            Giulia Galotto, Jeffrey P. Bibeau, and Luis Vidali

 

21. Live Cell Imaging of Arabidopsis Root Hairs

            Tijs Ketelaar

 

22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant

            Birgit Möller, Yvonne Poeschl, Sandra Klemm, and Katharina Bürstenbinder

 

23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls

            Takema Sasaki and Yoshihisa Oda

 

24. Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes

            Martin Potocký, Radek Bezvoda, and Přemysl Pejchar

 

25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to ‑Omics

            Petr Klíma, Vojtěch Čermák, Miroslav Srba, Karel Müller, Jan Petrášek, Josef Šonka, Lukáš Fischer, and Zdeněk Opatrný