Plant Cell Morphogenesis: Methods and Protocols

ISBN-13: 9781493960323 / Angielski / Miękka / 2016 / 285 str.

Viktor Arsky; Fatima Cvr Kova

Opis

This book features cutting-edge methods and protocols in plant cell morphogenesis in the MiMB standard format. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.

Recenzje

From the book reviews:

"A compendium of procedures and techniques for investigating the structure and shape of plant tissues and cells in a developmental context. ... I can certainly rate this book very useful for the scientific community, as it contains many valuable new techniques and inside knowledge. Furthermore, it could inspire researchers to use new technical approaches for their research topics and discuss these with the expert authors of these articles." (Kai R. Konrad, Journal of Plant Physiology, Vol. 171, 2014)

Spis treści

1. Essential  Methods of Plant Sample Preparation for Light Microscopy

Aleš Soukup and Edita Tylová

 

2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy

Aleš Soukup

 

3. Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues

Kieran J. D. Lee and J. Paul Knox

 

4. Automated Microscopy in Forward Genetic Screening of Arabidopsis

Tereza Dobisová and Jan Hejátko

 

5. Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections

Jana Albrechtová, Zuzana Kubínová, Aleš Soukup, and Jiří Janáček

 

6. Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells

Andreas Nebenführ

 

7. Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM

Amparo Rosero, Viktor Žárský, and Fatima Cvrčková

 

8. Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces

Dorota Kwiatkowska and Agata Burian

 

9. Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope

Olivier Hamant, Pradeep Das, and  Agata Burian

 

10. Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX

Pierre Barbier de Reuille, Sarah Robinson, and Richard S. Smith

 

11. Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy

Anne-Lise Routier-Kierzkowska and Richard S. Smith

 

12. High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy

Ichirou Karahara and Byung-Ho Kang

 

13. Reconstructing Plant Cells in 3D by Serial Section Electron Tomography

Kiminori Toyooka and Byung-Ho Kang

14. Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy

Jindřiška Fišerová and Martin W. Goldberg

 

15. Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections

Ilse Foissner and Margit Hoeftberger

 

16. Live Cell Imaging of Arabidopsis Root Hairs

Tijs Ketelaar

 

17. Morphological Analysis of Cell Growth Mutants in Physcomitrella

Luis Vidali and Jeffrey P. Bibeau

 

18. Plant Cell Lines in Cell Morphogenesis Research

Daniela Seifertová, Petr Klíma, Markéta Pařezová, Jan Petrášek, Eva Zažímalová, and Zdeněk Opatrný

 

19. Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes

Radek Bezvoda, Roman Pleskot, Viktor Žárský, and Martin Potocký

 

20. Lab-on-a-Chip for Studying Growing Pollen Tubes

Carlos G. Agudelo, Muthukumaran Packirisamy, and Anja Geitmann

 

21. Laser Microdissection of Plant Cells

Yvonne Ludwig and Frank Hochholdinger

 

22. Optical Trapping in Plant Cells

Tijs Ketelaar,  Norbert de Ruijter, and Stefan Niehren

 

23. Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery

Fatima Cvrčková and Michal Hála

 

Blurb

 Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on  classical light microscopy  and  histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized  electron microscopy  techniques, and methods  used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell  morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by  expert researchers in the field and  include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.