Plant Biotechnology and Medicinal Plants: Periwinkle, Milk Thistle and Foxglove

ISBN-13: 9783030229283 / Angielski / Twarda / 2019 / 202 str.

Mohamed Ramadan Rady

Spis treści

Chapter 1: Terpenoid indole alkaloid production from in vitro culture of Catharanthus roseus through biotic, abiotic elicitation and precursors feeding.

1-Abiotic elicitation and TIA production from in vitro cultures of C. roseus

1-1-Media composition and pH 

1-1-1-Media components

1-1-2-Medium pH

1-2- Growth regulators and phytohormones

1-2-1-Auxins and cytokinins

1-2-2-Methyl jasmonate

1-3-Light and ultraviolet (UV)-B light

1-3-1-Photoperiod regime

1-3-2-UV-B light

1-4-Temperature and deprivation of oxygen   

1-4-1- Temperature

1-4-2-Deprivation of oxygen

1-5- Biomass density and subculture cycles

1-5-1-Inoculum size

1-5-2-Subculture cycles

1-6-Bioregulators 

1-7-Permeabilizing agent and chemicals 

1-8-Long-term preservation

1-9-Small and high volume scale up 

Chapter 2: Biotic elicitation and TIA production from in vitro cultures of C.roseus

Chapter 3: Precursor feeding and TIA production from in vitro cultures of C.roseus

Chapter 4: Conclusion and future aspects

Sections on S. marianum and Digitalis will follow the same structural organization as described above for C. roseus

Blurb

Plant tissue culture and advanced biotechnologies have proven to be influential tools that complement conventional breeding and accelerate development of many medicinal plants.  Various approaches, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic elicitors and scaling up in bioreactors, have been explored to improve the production of secondary metabolites from different medicinal plants. This book provides a comprehensive description of various studies, carried out on in vitro culture and hairy root cultures of Catharanthus roseus, Silybum marianum and Digitalis species which have been considered as alternative sources for the production of anti-tumour compounds, flavonolignans and cardenolides. Specific focus is on elicitation strategy for increasing production of bioactive compounds of C. roseus L., S. marianum and Digitalis species to overcome the constrains of conventional propagation. This book is valuable for researchers or students working on medicinal plants, phytochemistry, and plant tissue culture. It also serves as a reference for the pharmaceutical industry.