Plant Biotechnology, Volume 1: Principles, Techniques, and Applications

ISBN-13: 9781771885805 / Angielski / Twarda / 2017 / 562 str.

Opis

This volume provides an informative tour of the basics of biotechnology to recent advances in biotechnology. It includes chapters on techniques of sterilization, anther/pollen culture, embryo culture and endosperm culture, callus induction, protoplast isolation and fusion as well as chapters on somaclonal variation and its approaches for crop improvement. Included are discussions on TILLING (targeting induced local lesions in genomes), advances in molecular techniques to study diversity, protein purification, and methods and analysis in protein-protein interaction detection. The volume also covers molecular markers and QTL mapping.

Spis treści

History of Biotechnology. Scope and Importance of Plant Biotechnology in Crop Improvement. Scope of Plant Biotechnology in the Developing Countries. Sterilization Technique. Basic Principles and Recent Advances in Anther/Pollen Culture for Crop Improvement. Embryo Culture and Endosperm Culture. Callus Induction. Protoplast Isolation and Fusion. Somaclonal Variation. Somaclonal Variation: A Tissue Culture Approach to Crop Improvement. Restriction Endonucleases. Ligation of Insert DNA in to Cloning Vector. Blotting Techniques. Advances in PCR Technology and RNA Interference. TILLING: Genome Poking with Diligences and Constraints. Advances in Molecular Techniques to Study Diversity. Protein Purification: Science and Technology. Protein–Protein Interaction Detection: Methods and Analysis. Molecular Markers in Plant Biotechnology. Development of Mapping Populations. Principles and Practices of Mapping QTLs in Plants. Association Mapping: A Tool for Dissecting the Genetic Basis of Complex Traits in Plants.