Plant Abiotic Stress Signaling

ISBN-13: 9781071630433 / Angielski / Twarda / 2023 / 486 str.

Ivan Couée

Opis

This volume provides conceptual strategies and methodological know-how over a wide range of stress situations that can be used as stepping stones to unravel the intricacies of abiotic stress signaling networks in plants. Chapters guide readers through achievements and challenges in the field and through up-to-date protocols covering identification of novel processes, validation of hypothetical mechanisms, and further characterization of currently-known pathways. Written in the format of the highly successful Methods in Molecular Biology series, wet-lab chapters include an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Abiotic Stress Signaling aims to be a comprehensive and innovative guide for students and researchers seeking to understand plant molecular mechanisms at the interface with environmental constraints and climate change.

Spis treści

 Part I: QUESTIONS and APPROACHES

 

1 Interplay of Methodology and Conceptualization in Plant Abiotic Stress Signaling

Ivan Couée

 

2 Complexity of Abiotic Stress Stimuli: Mimicking Hypoxic Conditions Experimentally on the Basis of Naturally Occurring Environments

Ailbhe Jane Brazel and Emmanuelle Graciet

 

3 Iron Availability and Homeostasis in Plants: a Review of Responses, Adaptive Mechanisms and Signaling

Nolenn Kermeur, Mathieu Pédrot, and Francisco Cabello-Hurtado

 

Part II. signalS AND SIGNALOMICS

 

4 Method for Calcium Live Imaging at Multi-Scales from Cellular to Organ Level in Arabidopsis thaliana

Carine  Alcon and Tou-Cheu Xiong

 

5 Functions of NO and H₂S Signal Molecules against Plant Abiotic Stress

Francisco J. Corpas and José M. Palma

 

6 Review of Lipid Biomarkers and Signals of Photooxidative Stress in Plants

Michel Havaux

 

7 The Plant Metabolic Changes and the Physiological and Signalling Functions in the Responses to Abiotic Stress

Feng Zhu, Yuming Sun, Sagar Sudam Jadhav, Yunjiang Cheng, Saleh Alseekh, and Alisdair R. Fernie

 

8 ¹⁵N-labelling of Leaves Combined with GC-MS Analysis as a Tool for Monitoring the Dynamics of Nitrogen Incorporation into Amino Acids

Anis M. Limami, Caroline Cukier, and Bertrand Hirel

 

9 Computational Metabolomics to Elucidate Molecular Signalling and Regulatory Mechanisms Associated with Biostimulant-Mediated Growth Promotion and Abiotic Stress Tolerance in Crop Plants

Kgalaletso Othibeng, Lerato Nephali, and Fidele Tugizimana

 

10 Electrical Signaling and its Functions under Conditions of Abiotic Stress: a Review of Methodological Approaches and Physiological Implications

Silke Lautner and Jörg Fromm

 

Part III. SIGNALING processes

 

11 Quantitative measurements of biochemical and molecular markers of oxidative stress signaling and responses

Graham Noctor, Mathias Cohen, Lug Trémulot, Frank Van Breusegem, and Amna Mhamdi

 

12 Fast identification of in vivo protein phosphorylation events using transient expression in leaf mesophyll protoplasts and Phos-tag^TM SDS-PAGE

Ellen Broucke, Filip Rolland, and Nathalie Crepin

 

13 Analysis of Plant L-Cysteine Desulfhydrase (LCD) Isozymes by Non-Denaturing Polyacrylamide Gel Electrophoresis

María A. Muñoz-Vargas, Marta Rodríguez-Ruiz, Salvador González-Gordo, José M. Palma, and Francisco J. Corpas

 

14 Metabolite-Based Genome-Wide Association Studies of Large-Scale Metabolome Analysis to Illustrate Alterations in the Metabolite Landscape of Plants upon Responses to Stresses

Feng Zhu, Mustafa Bulut, Yunjiang Cheng, Saleh Alseekh, and Alisdair R. Fernie

 

15 Deciphering Macromolecular Interactions Involved in Abiotic Stress Signaling: a Review of Bioinformatics Analysis

Gwenola Gouesbet

 

16 Multi-Omics Data Integration in the Context of Plant Abiotic Stress Signaling

Harold Duruflé and Sébastien Déjean

 

17 Protein-Protein Interactions in Abiotic Stress Signaling: an Overview of Biochemical and Biophysical Methods of Characterization

Ivan Couée and Gwenola Gouesbet

 

18 Beyond the Primary Structure of Nucleic Acids: Potential Roles of Epigenetics and Noncanonical Structures in the Regulations of Plant Growth and Stress Responses

Adriana Volná, Martin Bartas, Jakub Nezval, Radomír Pech, Petr Pečinka, Vladimír Špunda, and Jiří Červeň

 

Part IV. SYSTEMIC AND ECOSYSTEMIC SIGNALING

 

19 Laser microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses

Francisco R. Tadeo, Javier Agustí, Paz Merelo, and Manuel Talón

 

20 An Experimental Rhizobox System for the Integrative Analysis of Root Development and Abiotic Stress Responses under Water Deficit Conditions

Mickaël Durand, Amélie Morin, Benoît Porcheron, and Nathalie Pourtau

 

21 Live Whole-Plant Detection of Rapidly Accumulating Reactive Oxygen Species Following Applied Stress in Arabidopsis thaliana

Ronald J. Myers Jr., Sara I. Zandalinas, and Ron Mittler

 

22 Analysis of Small Non-Coding RNAs as Signaling Intermediates of Environmentally-Integrated Responses to Abiotic Stress

Christophe Penno, Julien Tremblay, Mary O’Connell Motherway, Virginie Daburon, Harriet Middleton, and Abdelhak El Amrani

 

23 Perspectives in Plant Abiotic Stress Signaling

Ivan Couée