Planarian Regeneration: Methods and Protocols

ISBN-13: 9781493978007 / Angielski / Twarda / 2018 / 573 str.

Jochen Rink

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Planarian Diversity and Phylogeny

Ronald Sluys and Marta Riutort

2. Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale

Jochen Rink

3. The Ecology of Freshwater Planarians

Miquel Vila-Farré and Jochen C. Rink

4. Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology

Sarah A. Elliott and Alejandro Sánchez Alvarado

5. Culturing Planarians in the Laboratory

M. Shane Merryman, Alejandro Sánchez Alvarado and James C. Jenkins

6. Total RNA Isolation from Planarian Tissues

Shang-Yun Liu and Jochen C. Rink

7. Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians

Markus A. Grohme, Miquel Vila-Farré, and Jochen C. Rink

8. Planarian High Molecular Weight DNA Isolation by Spooling

Shasha Zhang and Alejandro Sánchez Alvarado

9. Basic Histological Techniques for Planarians

Leigh Winsor and Ronald Sluys

10. Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians

David J. Forsthoefel, Kelly G. Ross, Phillip A. Newmark, and Ricardo M. Zayas

11. Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections

Teresa Adell, Sara Barberán, Miquel Sureda-Gómez, María Almuedo-Castillo, Nidia de Sousa, and Francesc Cebriá

12. Whole-Mount In Situ Hybridization of Planarians

Ryan S. King and Phillip A. Newmark

13. RNA In Situ Hybridization on Planarian Paraffin Sections

Jordi Solana

14. General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods

José María Martín-Durán

15. Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians

Li-Chun Cheng and Alejandro Sánchez Alvarado

16. Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL

Brad Stubenhaus and Jason Pellettieri

17. Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria

Carolyn E. Adler and Alejandro Sánchez Alvarado

18. RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA

Norito Shibata and Kiyokazu Agata

19. A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells

Tetsutaro Hayashi and Kiyokazu Agata

20. Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation

Irving E. Wang, Daniel E. Wagner, and Peter W. Reddien

21. Tissue Transplantations in Planarians

José I. Rojo-Laguna and Emili Saló

22. Live Imaging Planaria

Wei Shen, Yun Shen, Yun Wah Lam, and Danny Chan

23. Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques

John L. Brubacher, Ana P. Vieira, and Juliette Azimzadeh

24. Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC

Alexander Böser, Hannes C.A. Drexler, and Kerstin Bartscherer

25. Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method

Kannan Rangiah and Dasaradhi Palakodeti

O autorze

Jochen Rink, PhD
Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden, Germany
Tel: +49-(0)351-210-2435
Email: rink@mpi-cbg.de 

Blurb

This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.