Planar Chromatography - Mass Spectrometry

ISBN-13: 9781498705882 / Angielski / Twarda / 2015 / 380 str.

Teresa Kowalska; Mieczyslaw Sajewicz; Joseph Sherma

Opis

Planar Chromatography Mass Spectrometry focuses on a relatively new approach to chemical analysis in general, and to separation science in particular. It is the first book to systemically cover the theoretical background, techniques, instrumentation, and practical applications of planar chromatography mass spectrometry as a hyphenated tool of analytical chemistry. It also examines the high and as-yet unexploited potential of planar chromatography mass spectrometry for analytical use in scientific investigations. This book overviews the combination of planar chromatography, a relatively simple and cost-effective separation step for determining complex mixtures of compounds, with mass spectrometry, an efficient, highly instrumental, and relatively expensive technique that enables rapid identification of separated chemical species. It covers electrophoretic mass spectrometry methods and applications, which are considered planar chromatographic techniques and are increasingly being exploited in proteomic and molecular biology studies as well as for medical diagnostic purposes. It also provides a selection of applications, such as drug control and forensic and food analysis, including more difficult substances such as carbohydrates and lipids. The book advocates growth in using planar chromatography mass spectrometry in laboratories that have appropriate equipment but have not yet employed the techniques in combination. It also describes the use of a relatively inexpensive commercial system that can be adopted by laboratories currently working without the coupled methodology. Aiming to improve power and efficiency when other analytical methods are inadequate, Planar Chromatography Mass Spectrometry encourages separation science practitioners in academia and industry to combine the two methods for enhanced results."

Spis treści

Overview of the Field of TLC–MS and Contents of the Book. Applicability of Commercial and Noncommercial Thin Layers for Mass Spectrometric Detection. The Camag TLC–MS Interface. Principles of Mass Spectrometry Imaging Applicable to Thin-Layer Chromatography. Mass Spectrometry Applicable to Electrophoretic Techniques. Selection of Ionization Methods of Analytes in the TLC–MS Techniques. Interfacing TLC with Laser-Based Ambient Mass Spectrometry. TLC–MS Analysis Using Solvent Elution of Compounds from Chromatographic Media. Recording of Mass Spectra from Miniaturized Layers (UTLC–MS). Strategies of Coupling Planar Chromatography to HPLC–MS. Drug Analysis by TLC–DESI MS. Application of TLC and Plasma-Based Ambient MS in Bioanalytical Sciences. TLC/MALDI MS for the Analysis of Lipids. Application of TLC–MS to Drug Photodegradation Studies. Combination of Thin-Layer Chromatography with Laser Desorption Ionization– and Electrospray Ionization–Mass Spectrometric Techniques for Screening of Organic Compounds. Application of TLC–MS to Analysis of Drugs of Abuse. TLC–MS Analysis of Carotenoids, Triterpenoids, and Flavanols in Plant Extracts and Dietary Supplements. TLC/MALDI MS of Carbohydrates. Spontaneous Chiral Conversion and Peptidization of Amino Acids Traced by Means of TLC–MS.