Planar Cell Polarity: Methods and Protocols

ISBN-13: 9781617795091 / Angielski / Twarda / 2012 / 276 str.

Kursad Turksen

Opis

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states. In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world. As a volume in the highly successful Methods in Molecular Biology series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study.

Spis treści

1. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations

            Daryl J.V. David, Melanie A. McGill, R.F. Andrew McKinley, and Tony J.C. Harris

 

2. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell

            Bertrand Jauffred and Yohanns Bellaiche

 

3. Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus

            Yingqun Wang

 

4. Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity

            David Neff, Justin Hogan, and Simon Collier

 

5. Analysis of Cell Shape and Polarity during Zebrafish Gastrulation

            Douglas C. Weiser and David Kimelman

 

6. Analyzing Planar Cell Polarity during Zebrafish Gastrulation

            Jason R. Jessen

 

7. Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation

            Gun-Hwa Kim, Edmond Changkyun Park, and Jin-Kwan Han

 

8. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling

            Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, and Ira O. Daar

 

9. Gene Loss-of-Function and Live Imaging in Chick Embryos

            Anne C. Rios, Christophe Marcelle, and Olivier Serralbo

 

10. Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation

            Courtney Mezzacappa, Yuko Komiya, and Raymond Habas

 

11. Convergent Extension Analysis in Mouse Whole Embryo Culture

            Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D.E. Greene, and Andrew J. Copp

 

12. Analysis of PCP Defects in Mammalian Eye Lens

            Yuki Sugiyama and John W. McAvoy

 

13. Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea

            Helen May-Simera and Matthew W. Kelley

 

14. Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells

            Lisa Fujimura and Masahiko Hatano

 

15. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration

            Kristi LaMonica and Laura Grabel

 

16. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells

            Mitsuharu Endo, Michiru Nishita, and Yasuhiro Minami

 

17. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling

            Shinji Matsumoto and Akira Kikuchi

 

18. The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity

            Makoto Matsuyama and Akihiko Shimono

 

19. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models

            Daniel J. Jagger and Andrew Forge

 

20. Morphometric Analysis of Centrosome Position in Tissues

            Hester Happé, Emile de Heer, and Dorien J.M. Peters