Photosynthesis: Methods and Protocols

ISBN-13: 9781493977857 / Angielski / Twarda / 2018 / 355 str.

Sarah Covshoff

Spis treści

Part I: Measuring Photosynthetic Parameters

 

1. Survey of Tools for Measuring In Vivo Photosynthesis

            Berkley J. Walker, Florian A. Busch, Steven M. Driever, Johannes Kromdijk, and Tracy Lawson

 

2. Photosynthetic Gas Exchange in Land Plants at the Leaf Level

            Florian A. Busch

 

3. Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants

            Gavin M. George, Katharina Kölling, Roland Kuenzli, Matthias Hirsch-Hoffmann, Patrick Flütsch, and Samuel C. Zeeman

 

4. Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS)

            Qingfeng Song and Xin-Guang Zhu

 

5. Light-Response Curves in Land Plants

            Robert A. Coe and HsiangChun Lin

 

6. Chlorophyll Fluorescence on the Fast Timescale

            Olubukola O. Ajigboye, Rumiana V. Ray, and Erik H. Murchie

 

7. Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield

            Thomas J. Avenson and Aaron J. Saathoff

 

8. Chlorophyll Fluorescence Imaging

            Tracy Lawson and Silvere Vialet-Chabrand

 

9. Measurement of O₂ Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry

            Steven M. Driever and Neil R. Baker

 

10. Using Carbon Stable Isotopes to Study C₃ and C₄ Photosynthesis: Models and Calculations

            Nerea Ubierna, Meisha-Marika Holloway-Phillips, and Graham D. Farquhar

 

11. Liquid-Phase Measurements of Photosynthetic Oxygen Evolution

            Dmitriy Shevela, Wolfgang P. Schröder, and Johannes Messinger

 

Part II: Measuring Photosynthetic Enzyme Abundance and Catalytic Activity

 

12. Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting

            J. Alejandro Perdomo, Cristina R.G. Sales, and Elizabete Carmo-Silva

 

13. Extraction of Rubisco to Determine Catalytic Constants

            Douglas J. Orr and Elizabete Carmo-Silva

 

14. Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts

            Cristina R.G. Sales, Gustaf E. Degen, Anabela Bernardes da Silva, and Elizabete Carmo-Silva

 

Part III: Determining Cellular and Sub-Cellular Phenotypes

 

15. Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features

            Roxana Khoshravesh and Tammy L. Sage

 

16. Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves

            Vivien Rolland

 

17. 3D Clearing and Molecular Labeling in Plant Tissues

            William M. Palmer, Jamie R. Flynn, Antony P. Martin, Stephanie L. Reed, Christopher P.L. Grof, Rosemary G. White, and Robert T. Furbank

 

18. Evaluation of Lipids for the Study of Photosynthetic Membranes

            Helmut Kirchhoff and Robert Yarbrough

 

Part IV: Photosynthesis-Inspired Energy Generation

 

19. “Click” Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs

            Jackson D. Megiatto Jr. and Catia Ornelas

 

20. Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation

            Stephen J.L. Rowden, Paolo Bombelli, and Christopher J. Howe

Blurb

This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.