Phospholipid Signaling Protocols

ISBN-13: 9780896034914 / Angielski / Twarda / 1998 / 380 str.

Ian M. Bird

Opis

Cell membranes are not, as once believed, inert structures designed to contain the cell contents, but are in fact dynamic structures that are as me- bolically active as the cytosol and other cellular compartments they surround. Thus membranes not only contain mixtures of lipid and phospholipids, but also many proteins both embedded deeply within the membrane structure itself and also more loosely attached on the membrane surfaces. Though many such proteins have long been known to act as transport proteins, ion channels, hormone receptors, G proteins, cytoskeletal anchorage points, and so on, the major advance of recent years is the increasing understanding that the lipids and phospholipids in the membrane bilayer itself are also metabolized to b- logically active products that can diffuse either in the cytosol or in the m- brane bilayer to control the function of other proteins. Thus the concept of lipid-derived second messengers is now firmly established.

Recenzje

". . . the detailed information provided in Phospholipid Signaling Protocols makes it a welcome addition to an academic library that serves scientists actively working in the area of second messenger and membrane lipid chemistry. It is well representative of the previous volumes in the Methods in Molecular Biology series, and therefore is a worthwhile acquisition."-E-Streams

". . .this book is an extremely valuable resource for methodology in studying the molecular species of key phospholipid signaling molecules, as well as the enzymes that produce or degrade them. I personally have used several protocols in this book, and have not had any trouble adapting the methods to my own applications."-The Quarterly Review of Biology

"...gives an excellent overview of the most common techniques in use as well in biochemistry and molecular biology...The editor is to be congratulated for putting these diverse, very important protocols together..."-Cell Biology International 2000

"...does a very good job of covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signaling molecules."-Molecular Biotechnology

"...does a very good job covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signalling molecules." - Molecular Biotechnology

Spis treści

Part I. Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H] Phosphoinositides and [3H] Phosphoinositols Ian M. Bird Phosphoinositidase C Activation Assay II: Simple Analysis of Recovered Cellular Phosphoinositides and Phosphoinositols Ian M. Bird Phosphoinositidase C Activation Assay III: HPLC Analysis of Cellular Phosphoinositides and Phosphoinositols Ian M. Bird Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatography Flow Detection Lubo Zhang and Iain L. O. Buxton Preparation of [3H]Phosphoinositol Standards and Conversion of [3H] Phosphoinositides to [3H] Phosphoinositols Ian M. Bird Inositol 1,4,5-Trisphosphate Mass Assay Lubo Zhang Measurement of Cellular Diacylglycerol Content Wendy Bollinger Bollag and Richard D. Griner Phosphatidylinositol 4-Kinases: Assays and Product Analysis Rachel E. Meyers and Lewis C. Cantley Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Displacement Assay Jeroen van der Kaay, Pete J. Cullen, and C. Peter Downes Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography Gregory J. Parker, Joost C. Loijens, and Richard A. Anderson Determination of Phospholipase C- or Phospholipase D-Catalyzed Phosphatidylcholine Hydrolysis Michael J. O. Wakelam and Trevor R. Pettitt Measurement of Phospholipase D Activity Wendy Bollinger Bollag Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid Sandie I. Briand, Sylvie G. Bernier, and Gaétan Guillemette Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases Bryan A.Wolf Isotopic Efflux Studies as Indices of Phospholipase Activation Jacob Vadakekalam and Stewart Metz Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays Bruno Battistini, Serge Picard, Pierre Borgeat, and Pierre Sirois Measurement of Prostanoids, Leukotrienes, and Isoprostanes By Enzyme Immunoassays Bruno Battistini, Serge Picard, Pierre Borgeat, and Pierre Sirois Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways By High-Pressure Liquid Chromatography Pierre Borgeat, Serge Picard, Bruno Battistini, and Pierre Sirois Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis Pino Santana, Luisa F. Fanjul, and C. M. Ruiz de Galarreta Sphingomyelin and Ceramide Mass Assay Pino Santana, C. M. Ruiz de Galarreta, and Luisa F. Fanjul Sphingosine Kinase: Assay and Product Analysis Ana Olivera and Sarah Spiegel Analytical Methods and Steps to Sample Preparation For Determination of Molecular Species of Fatty Acids Sanda Clejan HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids In Diacylglycerol and Cellular Phospholipids Sanda Clejan Analysis of Molecular Species of Cellular Sphingomyelins and Ceramides Sanda Clejan Part II. General methods for monitoring changes in levels of key signaling pathway proteins and associated mRNA Solubilization and Assay of Cellular and Tissue Protein Cynthia E. Shaw Western Immunoblot Analysis Cynthia E. Shaw and Jing Zheng Immunohistochemistry and Immunocytochemistry Jing Zheng Extraction of Cellular and Tissue RNA Daniel S. Millican and Ian M. Bird Size Separation and Quantification of mRNA by Northern Analysis Ian M. Bird Preparation of

Blurb

In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry.

Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D. A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field.