4. Phospholipid Micelles for Peptide Drug Delivery
Karina Esparza, Dulari Jayawardena, and Hayat Onyuksel
5. Design, Preparation, and Characterization of Peptide-Based Nanocarrier for Gene Delivery
Mohsen Alipour and Saman Hosseinkhani
6. Gelatin Nanoparticles
Rania M. Hathout and Abdelkader A. Metwally
7. Green Synthesis of Chitosan-Silver/Gold Hybrid Nanoparticles for Biomedical Applications
Ibrahim M. El-Sherbiny and Mohammed Sedki
8. Methods of Fabrication of Chitosan-Based Nano-in-Microparticles (NMPs)
Ibrahim M. El-Sherbiny and Amr Hefnawy
9. Fabrication of Mucoadhesive-Dendrimers as Solid Dosage Forms
Nidhi Raval, Rahul Maheshwari, Kiran Kalia, and Rakesh Kumar Tekade
10. In Situ Vaccination of Tumors Using Plant Viral Nanoparticles
Abner A. Murray, Mee Rie Sheen, Frank A. Veliz, Steven N. Fiering, and Nicole F. Steinmetz
11. Bioconjugation in Drug Delivery: Practical Perspectives and Future Perceptions
Perihan Elzahhar, Ahmed S.F. Belal, Fatema Elamrawy, Nada A. Helal, and Mohamed Ismail Nounou
12. Conjugation of Triphenylphosphonium Cation to Hydrophobic Moieties to Prepare Mitochondria-Targeting Nanocarriers
Diana Guzman-Villanueva, Mark R. Mendiola, Huy X. Nguyen, Francis Yambao, Nusem Yu, and Volkmar Weissig
13. Surface Modification of Biomedically Essential Nanoparticles Employing Polymer Coating
Rahul Maheshwari, Nidhi Raval, and Rakesh Kumar Tekade
14. Peptide-Modified Gemini Surfactants: Preparation and Characterization for Gene Delivery
Mays Al-Dulaymi, Waleed Mohammed-Saeid, Anas El-Aneed, and Ildiko Badea
15. Preparation of Responsive Carbon Dots for Anticancer Drug Delivery
Tao Feng and Yanli Zhao
16. Surface Modification of Nanoparticles and Nanovesicles via Click Chemistry
Matthias Voigt, Thomas Fritz, Matthias Worm, Holger Frey, and Mark Helm
17. Polymersomes: Preparation and Characterization
Yumiao Hu and Liyan Qiu
18. Partially Polymerized Phospholipid Vesicles for Efficient Delivery of Macromolecules
Megha Goshi, Nicholas Pytel, and Tamer Elbayoumi
19. Fabrication of Nanostructured Lipid Carriers (NLC)-Based Gels from Microemulsion Template for Delivery through Skin
Medha D. Joshi, Rashmi H. Prabhu, and Vandana B. Patravale
20. Preparation and Characterization of Solid Lipid Nanoparticles-Based Gel for Topical Delivery
Vandana B. Patravale and Amit G. Mirani
21. Molecular-Level “Observations” of the Behavior of Gold Nanoparticles in Aqueous Solution and Interacting with a Lipid Bilayer Membrane
Priyanka A. Oroskar, Cynthia J. Jameson, and Sohail Murad
22. Atomic Force Microscopy for Cell Membrane Investigation
Mingjun Cai and Hongda Wang
23. Physico-Chemical Characterization of Phthalocyanine-Functionalized Quantum Dots by Capillary Electrophoresis Coupled to a LED Fluorescence Detector
Gonzalo Ramírez-García, Fanny d’Orlyé, Tebello Nyokong, Fethi Bedoui, and Anne Varenne
24. In Vitro Testing of Anticancer Nanotherapeutics Using Tumor Spheroids
Avanti Ganpule, Zishu Gui, Mohammed A. Almuteri, and Gerard G.M. D’Souza
This volume details protocols on formulation, surface modification, characterization, and application of a variety of pharmaceutical nanocarriers such as micelles, nanoparticles, dendrimers, carbon dots, polymersomes, and others. Chapters are targeted toward investigators working in academic and industrial laboratories conducting research in the broad field of pharmaceutical sciences, with an emphasis on drug delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Pharmaceutical Nanotechnology: Basic Protocols aims to be a source of inspiration to all investigators who are interested in the potential of the merger of nanotechnology with pharmaceutical sciences.