Phage Display

ISBN-13: 9781071633809 / Angielski / Twarda / 2023

Opis

This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phage Display: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.  

Spis treści

PART I:         INTRODUCTION

1. Antibody Phage Display

Alia Nur, Maren Schubert, Jing Yi Lai, Michael Hust, Yee Siew Choong, Wan Yus Haniff Wan Isa, and Theam Soon Lim

 

PART II:        CONSTRUCTION OF ANTIBODY PHAGE DISPLAY LIBRARIES

 

2. Construction of human immune and naive scFv Phage Display libraries

Maximilian Ruschig, Philip Alexander Heine, Viola Fühner, Kilian Johannes Karl Zilkens, Stephan Steinke, Maren Schubert, Federico Bertoglio, and Michael Hust

 

3. Construction of Naïve and Immune Human Fab Phage Display Library  

Jing Yi Lai and Theam Soon Lim

 

4. Construction of synthetic antibody phage display libraries

Kim Anh Giang, Sachdev S. Sidhu, and Johan Nilvebrant

 

5. Construction of chicken and ostrich antibody libraries

Jeanni Fehrsen, Susan Wemmer and Wouter van Wyngaardt

 

6. Construction of Rabbit Immune Antibody Libraries

Thi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim

  7. Isolation and characterization of single-domain antibodies from immune phage display libraries

Martin A. Rossotti, Frederic Trempe, Henk van Faassen, Greg Hussack, and Mehdi Arbabi-Ghahroudi

 

8. Phagekines: directed evolution and characterization of functional cytokines displayed on phages

Gertrudis Rojas, Tania Carmenate, Gisela García-Pérez, Dayana Pérez-Martínez

9. Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly 

 Ashley K. Grahn, Grace L. Allen, and Brian K. Kay 

 

10. Construction of an ultra-large phage display library by Kunkel mutagenesis and rolling circle amplification

Renhua R. Huang, Michael Kierny, Veronica Volgina, Makio Iwashima, Christina Miller and Brian K. Kay

11. Construction of semi-synthetic shark vNAR yeast surface display antibody libraries

Harald Kolmar, Julius Grzeschik, Doreen Könning, Simon Krah, and Stefan Zielonka

 

PART III:      SELECTION STRATEGIES FOR ANTIBODIES

12. Antibody selection via Phage Display in microtiter plates

Stephan Steinke, Kristian Daniel Ralph Roth, Maximilian Ruschig, Nora Langreder, Saskia Polten, Kai-Thomas Schneider, Rico Ballmann, Giulio Russo, Kilian Johannes Karl Zilkens, Maren Schubert, Federico Bertoglio, and Michael Hust

 

13. Antibody selection in solution using magnetic beads
Philip Alexander Heine, Maximilian Ruschig, Nora Langreder, Esther Veronika Wenzel, Maren Schubert, Federico Bertoglio and Michael Hust

 

14. Streptavidin Coated Solid Phase Extraction (SPE) Tips For Antibody Phage Display Biopanning  

Theam Soon Lim, Angela Chiew Wen Ch’ng, Brenda Pei Chui Song and Jing Yi Lai

 

15. Magnetic Nanoparticle-Based Semi-Automated Panning for High-throughput Antibody Selection

Angela Chiew Wen Ch’ng, Zoltán Konthur and Theam Soon Lim

 

16. Antibody selection on cells targeting membrane proteins

Viktor Glaser, Ümran Karsli, Maike Hagedorn, and Tom Pieper

 

17. Targeting Intracellular Antigens With pMHC-binding Antibodies: A Phage Display Approach

Zhiyuan Yang, Zhihao Wu, Brian H. Santich, Jingbao Liu, Cheng Liu, and Nai-Kong V. Cheung

 

18. Antibody Isolation from a Human Synthetic Libraries of Single-chain Antibodies and analysis using NGS

Adi Amir, David Taussig, Almog Bitton, Limor Nahary, Anna Vaisman-Mentesh, Itai Benhar, and Yariv Wine

 

19. Selection of affibody affinity proteins from phagemid libraries

Kim Anh Giang, Per-Åke Nygren, and Johan Nilvebrant

 

PART IV:        COMPLEMENTARY APPROACHES FOR ANTIBODY PHAGE DISPLAY SELECTIONS

 

20. Antibody affinity and stability maturation by error-prone PCR

Nora Langreder, Dorina Schäckermann, Tobias Unkauf, Maren Schubert, André Frenzel, Federico Bertoglio, and Michael Hust

 

21. Antibody batch cloning

Rico Ballmann, Kai-Thomas Schneider, Kristian Daniel Ralph Roth, and Stefan Dübel

 

22. Deep Mining of Complex Antibody Phage Pools

Tulika Tulika and Anne Ljungars

 

23. High-throughput IgG reformatting and expression using hybrid secretion signals and InTag positive selection technology

Georgina Sansome, Veronika Rayzman, Irene Kiess, Michael J. Wilson, Con Panousis and Chao-Guang Chen

 

24. Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols

Mary R. Ferguson, Qiana M. Mendez, Felicity E. Acca, Cassandra D. Chapados, Holland A. Driscoll, Kezzia S. Jones, Gregory Mirando, Michael Weiner,and Xiaofeng Li

 

25. Mapping polyclonal antibody responses to infection using next generation phage display

 

Maria T. Tsoumpeli, Anitha Varghese, Jonathan P. Owen, Ben C. Maddison, Janet M. Daly,and Kevin C. Gough

 

26. Applications of high-throughput DNA sequencing to  single-domain antibody discovery and engineering

Michael J. Lowden, Eric K. Lei, Greg Hussack, and Kevin A. Henry

 

PART V:        EPITOPE MAPPING AND BIOMARKER DISCOVERY BY PHAGE DISPLAY

 

27. Biomarker discovery by ORFeome Phage Display

Philip Alexander Heine, Rico Ballmann, Praveen Thevarajah, Giulio Russo, Gustavo Marçal Schmidt Garcia Moreira, and Michael Hust

 

28. Mapping Epitopes by Phage Display

Stephan Steinke, Kristian Daniel Ralph Roth, Ruben Englick, Nora Langreder, Rico Ballmann, Viola Fühner, Kilian Johannes Karl Zilkens, Gustavo Marçal Schmidt Garcia Moreira, Allan Koch, Filippo Azzali, Giulio Russo, Maren Schubert, Federico Bertoglio, Philip Alexander Heine, and Michael Hust

 

29. Epivolve: A Protocol for Site-directed Antibodies

Xiaofeng Li, Kezzia S. Jones_, Felicity E. Acca, Cassandra D. Chapados, Holland A. Driscoll, Emily P. Fuller, Qiana M. Mendez, Gregory Mirando, Michael P. Weiner and Mary R. Ferguson