Permeability Barrier: Methods and Protocols

ISBN-13: 9781071616758 / Angielski / Miękka / 2022

Turksen, Kursad

Opis

This detailed edition reflects the significant new findings in the components of permeability barriers and how they work in different tissues with a collection of cutting-edge techniques. Chapters explore the formation, maintenance, regulation, and dynamics of permeability barriers in an effort to push the boundaries of the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Permeability Barrier: Methods and Protocols, Second Edition serves as an invaluable guide for both experts but novices in the stem cell field and other related areas of research.

Spis treści

1. Use of Ussing Chambers to Measure Paracellular Permeability to Macromolecules in Mouse Intestine

            Doriane Aguanno, Barbara Graziela Postal, Véronique Carrière, and Sophie Thenet

 

2. Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line

            Barbara Graziela Postal, Doriane Aguanno, Sophie Thenet, and Véronique Carrière

 

3. Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium

            Shigeru Yamada and Yasunari Kanda

 

4. Selective Regional Isolation of Brain Microvessels

            Fernanda Medina-Flores, Gabriela Hurtado-Alvarado, and Beatriz Gómez-González

 

5. Estimating Brain Permeability Using In Vitro Blood-Brain Barrier Models

            Saeideh Nozohouri, Behnam Noorani, Abraham Al-Ahmad, and Thomas J. Abbruscato

 

6. In Vitro Human Blood-Brain Barrier Model for Drug Permeability Testing

            Ece Bayir and Aylin Sendemir

 

7. Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase

            Bulent Ahishali and Mehmet Kaya

 

8. Blood-Brain Barrier (BBB) Permeability and Transport Measurement In Vitro and In Vivo

            Bingmei M. Fu, Zhen Zhao, and Donghui Zhu

 

9. Assessment of Blood-Brain Barrier Permeability Using Miniaturized Fluorescence Microscopy in Freely-Moving Rats

            Jeffrey L. Barr, G. Cristina Brailoiu, Ellen M. Unterwald, and Eugen Brailoiu

 

10. Measurement of Lung Vessel and Epithelial Permeability In Vivo with Evans Blue

            Prestina Smith, Lauren A. Jeffers, and Michael Koval

 

11. Measurement of Airway Epithelial Permeability: Methods and Protocols

            Hasan Yüksel and Merve Öcalan

 

12. Vascular Permeability Assays In Vivo

            Mir S. Adil and Payaningal R. Somanath

 

13. Endothelial Permeability Assays In Vitro

            Mir S. Adil and Payaningal R. Somanath

 

14. Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells

            Mikkel R. Holst, Simone S.E. Nielsen, and Morten S. Nielsen

 

15. An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity

            Siwen Wu, Lingling Wang, Elizabeth I. Tang, Junlu Wang, and C. Yan Cheng

 

16. Ussing Chamber Methods to Study the Esophageal Epithelial Barrier

            Solange M. Abdulnour-Nakhoul and Nazih L. Nakhoul

 

17. A Simple Method to Test Mechanical Strain on Epithelial Cell Monolayers Using a 3D-Printed Stretcher

            Amanda C. Daulagala, John Yost, Amirreza Yeganegi, William J. Richardson, Michael J. Yost, and Antonis Kourtidis

 

18. Differentiating between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo

            Sandra D. Chanez-Paredes, Shabnam Abtahi, Wei-Ting Kuo, and Jerrold R. Turner

 

19. Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly

            Pawin Pongkorpsakol, Wilasinee Satianrapapong, Preedajit Wongkrasant, Peter R. Steinhagen, Nuttha Tuangkijkul, Nutthapoom Pathomthongtaweechai, and Chatchai Muanprasat

 

20. A Method to Prepare a Bioprobe for Regulatory Science of the Drug Delivery System to the Brain: An Angulin Binder to Modulate Tricellular Tight Junction-Seal

            Keisuke Tachibana and Masuo Kondoh

 

21. dSTORM Imaging and Analysis of Desmosome Architecture

            Reena R. Beggs, William F. Dean, and Alexa L. Mattheyses