Performing Enzymatic Reactions Using Novel Polymer-Bound ATP

ISBN-13: 9783844309805 / Angielski / Miękka / 2011 / 120 str.

Wael Abdelmoez

Opis

the present work was devoted to develop an efficient synthetic process which requires cofactors. The enzymatic glucose 6-phosphate (G6P) production from glucose was demonstrated as a model process. As cofactors, two new polymer-bound adenine nucleotides (ATP and ADP) were synthesized using starburst polyamidoamine (PAMAM) dendrimers. Then, the kinetics of the reactions catalyzed by Bacillus stearothermophilus glucokinase (GK) and acetate kinase (AK) using these polymer-bound cofactors were studied extensively. Finally, the production of G6P using the polymer-bound ATP in a plug flow-type hollow-fiber membrane reactor (PFMR) in a diffusion mode as well as a continuous stirred tank-type membrane reactor (CSTMR) in a filtration mode was investigated theoretically

O autorze

Associate Professor at Chemical Engineering Deprtment, Minia University, Egypt. Dr. Abdelmoez mixed between the academia and the professional life through being an industrial consultant for many factories. In 2003, he obtained his PhD from Japan at Osaka Prefecture University then, he worked there for another 5 years at the COE project.