Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization

ISBN-13: 9781493930333 / Angielski / Twarda / 2015 / 368 str.

Andras Nagy; Kursad Turksen

Opis

This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases. Specifically, representative protocols on various disease models have been collected from labs around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization is an ideal reference for scientists working on furthering iPS research.

Spis treści

1. Generation of Patient-Specific Induced Pluripotent Stem Cell from Peripheral Blood Mononuclear Cells by Sendai Reprogramming Vectors

            Oscar Quintana-Bustamante and Jose C. Segovia

 

2. A Doxycycline-Inducible System for Genetic Correction of iPSC Disease Models

            Xiuli Sim, Fabian L. Cardenas-Diaz, Deborah L. French, and Paul Gadue

 

3. Generation and Characterization Of Patient-Specific Induced Pluripotent Stem Cell For Disease Modeling

            Renuka Sivapatham and Xianmin Zeng

 

4. Modeling Genomic Imprinting Disorders Using Induced Pluripotent Stem Cells

            Stormy J. Chamberlain, Noelle D. Germain, Pin-Fang Chen, Jack S. Hsiao, and Heather Glatt-Deeley

 

5. Generation and Characterization of Induced Pluripotent Stem Cells from Patients with mtDNA Mutations

            Riikka H. Hämäläinen and Anu Suomalainen

 

6. Skin Biopsy and Patient-Specific Stem Cell Lines

            Yao Li, Huy V. Nguyen, and Stephen H. Tsang

 

7. Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells

            Emi Shoji, Knut Woltjen, and Hidetoshi Sakurai

 

8. Using Human Induced Pluripotent Stem Cells to Model Skeletal Diseases

            Emilie Barruet and Edward C. Hsiao

 

9. Modeling Cardiovascular Diseases with Patient-Specific Human Pluripotent Stem Cell-Derived Cardiomyocytes

            Paul W. Burridge, Sebastian Diecke, Elena Matsa, Arun Sharma, Haodi Wu, and Joseph C. Wu

 

10. Calcium Imaging in Pluripotent Stem Cell-Derived Cardiac Myocytes

            Anna Walter, Tomo Šarić, Jürgen Hescheler, and Symeon Papadopoulos

 

11. Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization of Cardiac Cells

            Fabian Zanella and Farah Sheikh

 

12. Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes Under Defined Conditions

            Cathelijne W. van den Berg, David A. Elliott, Stefan R. Braam, Christine L. Mummery, and Richard P. Davis

 

13. Generation of Cardiomyocytes from Pluripotent Stem Cells

            Hiroko Nakahama and Elisa Di Pasquale

 

14. Generation and Characterization of Patient-Specific iPSC-Model for Cardiovascular Disease

            Yee Ki Lee, X. Ran, K.W.H. Lai, V.Y.M. Lau, D.C.W. Siu, and H.F. Tse

 

15. Transgene-Free Disease-Specific iPSC Generation from Fibroblasts and Peripheral Blood Mononuclear Cells

            Kerem Fidan, Ayyub Ebrahimi, Özlem H. Çağlayan, Burcu Özçimen, and Tamer T. Önder

 

16. Generation and Neuronal Differentiation of Patient-Specific Induced Pluripotent Stem Cells Derived from Niemann-Pick Type C1 Fibroblasts

            Michaela Trilck, Rayk Hübner, and Moritz J. Frech

 

17. Multisystemic Disease Modeling of Liver-Derived Protein Folding Disorders Using Induced Pluripotent Stem Cells (iPSCs)

            George James Murphy and Amy Leung

 

18. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human Induced Pluripotent Stem Cells

            Lipeng Tian, Neha Prasad, and Yoon-Young Jang

 

19. Generation of Human Induced Pluripotent Stem Cells Using RNA-Based Sendai Virus System and Pluripotency Validation of the Resulting Cell Population

            Valeria Chichagova, Irene Sanchez-Vera, Lyle Armstrong, David Steel, and Majlinda Lako

 

20. Modeling Axonal Phenotypes with Human Pluripotent Stem Cells

            Kyle R. Denton, Chong-Chong Xu, and Xue-Jun Li

 

21. Mitochondrial Disease-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization

            Xuan Zhang, Shishi Li, Wei Yang, Huaye Pan, Dajiang Qin, Xufen Zhu, and Qingfeng Yan

 

22. Patient-Specific Induced Pluripotent Stem Cell Models: Characterization of iPS Cell-Derived Cardiomyocytes

            Toru Egashira, Shinsuke Yuasa, Shugo Tohyama, Yusuke Kuroda, Tomoyuki Suzuki, Tomohisa Seki, and Keiichi Fukuda

 

23. Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions

            Sara Caxaria, Susanne Arthold, Amit C. Nathwani, and Pollyanna Agnes Goh