Patient-Derived Mouse Models of Cancer: Patient-Derived Orthotopic Xenografts (Pdox)

ISBN-13: 9783319574233 / Angielski / Twarda / 2017 / 296 str.

Robert M. Hoffman

Opis

This text highlights seminal discoveries and also provides comprehensive and state-of the-art approach to mouse models of human patient tumors. These areas include training, basic techniques, as well as general troubleshooting. Subsequent chapters focus on the different mouse models of patient tumors including the various strains of immunodeficient mice currently available and the transplantation techniques that can be used as well as state-of-the-art imaging techniques. Practical applications of the models from drug discovery, genome analysis to personalized treatment are also covered. Written by experts in that field, each of these sections address these critical issues. A brief review of the existing literature addressing the particular topic follows in each section.
Presently, there is no single source to provide information on technique and uses of mouse models of human patient tumors. Patient-Derived Mouse Models of Cancer will satisfy this need for cancer researchers, oncologists, pharmaceutical and biotechnology industry scientists as well as molecular biologists studying in vivo systems

Spis treści

1. In memorium:  Jorgen Rygaard (1934-2016) Father of patient derived mouse models of cancer and modern cancer research.

2. The revival of patient-derived mouse models of cancer:  way back to the future

3. Patient-derived xenograft models for human cancer, The Freiburg experience

4. From Ectopic to Orthotopic Tumor Grafting Sites: Evidence for a Critical Role of the Host Tissue Microenvironment for the Actual Expression of the Malignant Phenotype

5. The Effects of the Organ Microenvironment on Metastatic-Cell Gene Signatures

6. Techniques for surgical orthotopic implantation of human tumors to immunodeficient mice

7. The first patient-derived orthotopic xenograft (PDOX) mouse models of cancer: Cancer of the colon, pancreas, lung, breast, ovary, and mesothelioma

8. Patient-derived xenograft models of prostate cancer

9. Patient-Derived Mouse Models of Sarcoma

10. Cervical cancer patient-derived orthotopic xenograft (PDOX) models

11. The use of pediatric patient-derived xenografts for identifying novel agents and combinations

12. Development of Orthotopic and Spontaneous Metastatic Human Tumor Xenograft Models for Experimental Therapeutics

13. Use of patient-derived orthotopic xenografts (PDOX) to evaluate transformative cancer therapeutics

14. Fluorescent protein-expressing transgenic nude mice as hosts for patient tumors

15. Fluorescence imaging of tumors in human patient-derived orthotopic xenograft (PDOX) mouse models:  The iPDOX model

16. The use of patient-derived orthotopic xenograft (PDOX) models to develop curative fluorescence-guided surgery of cancer

17. Molecular characteristics of patient-derived tumor xenografts: genomic similarity to patient tumors and relevance for biomarker discovery

18. Complementarity of patient-derived orthotopic xenografts (PDOX) models and molecular profiling for optimal therapy

19. 3D Cell Culture Models

20. Why patient derived mouse models need to be orthotopic

21. Foreward: The future of patient-derived models of cancer

O autorze

R.M. Hoffman has been a cancer researcher since 1965.  Dr. Hoffman is the pioneer of the patient-derived orthotopic xenograft (PDOX) mouse model, which is featured in the present volume.  Dr. Hoffman is a pioneer in many areas of cancer research described in his almost 1,000 scientific publications that have been cited more than 35,000 times with an h-index of almost 100.  Dr. Hoffman is currently a Professor of Surgery at the University of California San Diego and Founding President of AntiCancer Inc.