Patch Clamp Techniques: From Beginning to Advanced Protocols

ISBN-13: 9784431561057 / Angielski / Miękka / 2016 / 439 str.

Yasunobu Okada

Opis

Patch-clamping is a unique technique that enables real-time measurements of ion channel functions at the single-molecule level. This volume provides an overview of modern patch clamp techniques.

Recenzje

From the reviews:

"This book is the most current single 'how to' manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. ... The patch techniques for brain and spinal slices in culture has several chapters in this book. I recommend this to all those with an interest in biochemistry, membrane biology, neurophysiology, neurologists and neurosurgeons." (Joseph J. Grenier, Amazon.com, May, 2014)

Spis treści

Ch. 1          Prologue: The Ion Channel

Ch. 2          Patch-Clamp Techniques: General Remarks

Ch. 3          Whole-Cell Patch Method

Ch. 4          Perforated Whole-Cell Patch-Clamp Technique: A User’s Guide

Ch. 5          Methods for Processing and Analyzing Single-Channel Data

Ch. 6          Channel Noise

Ch. 7          Slice Patch Clamp

Ch. 8          Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms

Ch. 9          Analysis of Synaptic Plasticity with the Slice Patch-Clamp Recording Technique

Ch. 10        Patch-Clamp Recordings from Neuronal Dendrites

Ch. 11        In Vivo Blind Patch-Clamp Recording Technique

Ch. 12        Two-Photon Targeted Patch-Clamp Recordings in Vivo

Ch. 13        Transporter Current Measurements

Ch. 14        Giant Patch and Macro Patch

Ch. 15        Pipette Perfusion Technique

Ch. 16        Planar Lipid Bilayer Method for Studying Channel Molecules

Ch. 17        Patch-Clamp Capacitance Measurements

Ch. 18        Brief Guide to Patch-Clamp Current Measurements in Organelle Membranes

Ch. 19        Role of Ion Channels in Plants

Ch. 20        Automated Patch Clamping

Ch. 21        Patch-Clamp Biosensor Method

Ch. 22        Temperature-Evoked Channel Activation: Simultaneous Detection of Ionic Currents and Temperature

Ch. 23        Heterologous Expression Systems and Analyses of Ion Channels

Ch. 24        Patch-Clamp and Single-Cell Reverse Transcription-Polymerase Chain Reaction/Microarray Analysis

Ch. 25        Smart-Patch Technique

Ch. 26        Ion Channel Pore Sizing in Patch-Clamp Experiments

Ch. 27        Digital Recordings of Patch-Clamp Data

Ch. 28        Solutions for Patch-Clamp Experiments