Patch-Clamp Methods and Protocols

ISBN-13: 9781588296986 / Angielski / Twarda / 2007 / 336 str.

Peter Molnar; James J. Hickman

Opis

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training, and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is organized by the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. Individual techniques are explored within the area that they are applied most often. Researchers will find this text to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

Recenzje

From the reviews:

"This book will be helpful to researchers already working in the field of electrophysiology, but also those coming to this field from pharmacological, physiological, and biophysical areas." - Doody's Book Review Service

"'Patch-clamp: Methods and Protocols' introduces some common patch-clamp applications. It is intended to assist scientists in identifying problems, which could be best addressed by this technique. The book provides the step-by-step procedures to perform the experiments that answer those questions. ... It will be a useful companion for moderately advanced users wishing to extend their skills." (Maria Drigelova, General Physiology and Biophysics, Vol. 27, April, 2008)

Spis treści

Table of contents Part 1. Pharmacology 1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA receptors using whole-cell patch clamp method. Laszlo Fodor and Jozsef Nagy 2. Memantine as an example of a fast, voltage-dependent, open channel N-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and Kate Gilling 3. Methods for evaluation of positive allosteric modulators of glutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and Eric S. Nisenbaum 4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia and Jia Xu 5. Flip-the-Tip: automated patch clamping based on glass electrodes. Michael Fejtl, Uwe Czubayko, Alexander Hümmer, Tobias Krauter and Albrecht Lepple-Wienhues 6. The Roboocyte: Automated Electrophysiology Based on Xenopus Oocytes. Christine Leisgen, Mike Kuester and Christoph Methfessel Part 2. Physiology 7. Infrared guided laser stimulation as a tool for elucidating the synaptic site of expression of long-term synaptic plasticity. Gerhard Rammes, Mathias Eder, Walter Zieglgänsberger and Hans-Ulrich Dodt 8. Single-cell RT-PCR, a Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity. Maria Toledo-Rodriguez and Henry Markram 9. Mechanosensitive Ion Channels investigated simultaneously by Scanning Probe Microscopy and Patch clamp. Matthias G. Langer 10. Synaptic Connectivity in Engineered Neuronal Networks. Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J. Hickman 11. Modeling of Action Potential Generation in NG108-15 Cells. Peter Molnar and James J. Hickman 12. Whole-cell Voltage-clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique. Sandrine Pouvreau, Claude Collet, Bruno Allard and Vincent Jacquemond 13. Determination of channel properties at the unitary level in adult mammalian isolated cardiomyocytes. RomainGuinamard 14. Electrophysiological Properties of Embryonic Stem Cells During Differentiation into Cardiomyocyte-like Cell Types. Antoni C.G. van Ginneken and Arnoud. C. Fijnvandraat 15. Hybrid Neuronal Network Studies Under Dynamic Clamp. Alan D. Dorval II, Jonathan Bettencourt, Theoden I. Netoff and John A. White 16. Cardiac channelopathies studied with the dynamic action potential clamp technique. Géza Berecki, Jan G. Zegers, Ronald Wilders and Antoni C.G. van Ginneken Part 3. Biophysics 17. Principles of Single-Channel Kinetic Analysis. Feng Qin * Theoretical chapter 18. Use of Xenopus oocytes to measure ionic selectivity of pore-forming peptides and ion channels. Thierry Cens and Pierre Charnet 19. Estimation of quantal parameters with multiple-probability fluctuation analysis. Chiara Saviane and Robin Angus Silver

Blurb

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.