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Pancreatic Cancer: Methods and Protocols
ISBN-13: 9781493959822 / Angielski / Miękka / 2016 / 389 str.
Pancreatic Cancer: Methods and Protocols
ISBN-13: 9781493959822 / Angielski / Miękka / 2016 / 389 str.
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This book offers a range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It features step-by-step detail essential for reproducible results and implementation advice from the experts.
From the reviews of the second edition:
"This book describes methods and techniques that are used in pancreatic cancer research. ... It will be most useful for postdoctoral researchers and novice principal investigators, as well as any investigators who want to focus on pancreatic cancer research, particularly if they are looking for a technique to fit a specific need. ... It is a useful reference and go-to guide for exploring new protocols and techniques. It is also handy for seeing how an existing technique might be altered slightly to suit another need." (Naomi Akagi, Doody's Book Reviews, September, 2013)1. Identification and Analysis of Precursors to Invasive Pancreatic Cancer
Hanno Matthaei, Marco Dal Molin, Anirban Maitra,
2. Tissue Microarrays: Construction and Use
Helen Remotti
3. Immunohistochemistry of Pancreatic Neoplasia
Sukhwinder Kaur, Tomohiro Shimizu, Michael J. Baine, Sushil Kumar and
Surinder K. Batra 4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer Lorenzo F. Sempere, and Murray Korc 5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications Jaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis, Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova 6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis Alvin Makohon-Moore and Christine A. Iacobuzio-Donahue 7. DNA Methylation Analysis in Human Cancer Eileen O’Sullivan, Michael Goggins 8. Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients Michael J. Baine, Kavita Mallya, and Surinder K. Batra 9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee 10. The Prevention and Genetics of Pancreatic Cancer – A Programmatic Approach Aimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht 11. Development of Orthotopic Pancreatic Tumor Mouse Models Wanglong Qiu, Gloria H. Su 12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems Emman Mascariñas, Guido Eibl , Paul J. Grippo 13. Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound Stephen A. Sastra and Kenneth P. Olive 14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells Yuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi 15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo Chen Huang and Keping Xie 18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells Richard D. Dinnen, Yuehua Mao, and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor Angiogenesis Minji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi Screenings Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva
4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer
Lorenzo F. Sempere, and Murray Korc
5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications
Jaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis, Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova
6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis
Alvin Makohon-Moore and Christine A. Iacobuzio-Donahue
7. DNA Methylation Analysis in Human Cancer
Eileen O’Sullivan, Michael Goggins
8. Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients
Michael J. Baine, Kavita Mallya, and Surinder K. Batra 9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee 10. The Prevention and Genetics of Pancreatic Cancer – A Programmatic Approach Aimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht 11. Development of Orthotopic Pancreatic Tumor Mouse Models Wanglong Qiu, Gloria H. Su 12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems Emman Mascariñas, Guido Eibl , Paul J. Grippo 13. Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound Stephen A. Sastra and Kenneth P. Olive 14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells Yuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi 15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo Chen Huang and Keping Xie 18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells Richard D. Dinnen, Yuehua Mao, and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor Angiogenesis Minji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi Screenings Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva
9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine
Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee
10. The Prevention and Genetics of Pancreatic Cancer – A Programmatic Approach
Aimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht
11. Development of Orthotopic Pancreatic Tumor Mouse Models
Wanglong Qiu, Gloria H. Su
12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems
Emman Mascariñas, Guido Eibl , Paul J. Grippo
13. Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound
Stephen A. Sastra and Kenneth P. Olive
14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells
Yuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi 15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo Chen Huang and Keping Xie 18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells Richard D. Dinnen, Yuehua Mao, and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor Angiogenesis Minji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi Screenings Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva
15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells
Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach
16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis
Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang
17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo
Chen Huang and Keping Xie
18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells
Richard D. Dinnen, Yuehua Mao, and Robert L. Fine
19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods
Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor Angiogenesis Minji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi Screenings Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva
20. Evaluating Tumor Angiogenesis
Minji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi Screenings Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva
21. Pooled Genome-wide RNAi Screenings
Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva
22. Pooled ShRNA screenings: Computational Analysis
Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva
Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA. Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes. The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.
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