PCR Technology: Current Innovations, Third Edition

ISBN-13: 9781439848050 / Angielski / Twarda / 2013 / 457 str.

Tania Nolan

Opis

A technique used to amplify the number of copies of a specific region of DNA, Polymerase Chain Reaction (PCR) is the forefront of the dramatic development of biochemistry.

Spis treści

Template Preparation. Pre-PCR Processing Strategies. Use of a Liposomal Internal Control Vehicle for Whole-Process Quality Assurance of Nucleic Acid Amplification. Reaction Components. Oligonucleotide Synthesis and Purification. Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction. Real-Time Fluorescent PCR by Labeled Primer with a Single Fluorescent Molecule. Using Scorpion Primers for Genotyping. LNA: Adding New Functionality to PCR. Modified dNTPs: A Toolbox for Use in PCR. Instruments. Thermocycler Calibration and Analytical Assay Validation. Ultra-High-Speed PCR Instrument Development. Dropletization of Bioreactions: How Single-Cell Quantitative Analyses May Improve Gene Measurement and Cancer Diagnosis. Design, Optimization, QC, and Standardization. Assay Design for Real-Time qPCR. Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension. Development and Use of qPCR Assays for Detection and Study of Neglected Tropical and Emerging Infectious Diseases. MIQE: Guidelines for Reliable Design and Transparent Reporting of Real-Time PCR Assays. Data Analysis. Hypothesis-Driven Approaches to Multivariate Analysis of qPCR Data. Experiment Design, Data Management, and Univariate Statistical Analysis of Gene-Expression Data Obtained by Real-Time Quantitative PCR. Biomarker Discovery via RT-qPCR and Bioinformatical Validation. Optimization of Quantitative Real-Time PCR for Studies in Cartilage Mechanobiology. Applications. Enriching DNA Sequences with Nucleotide Variation by Thymidine Glycosylases Combined with Suppression PCR. Chromosome Walking by Inverse PCR. Expression Profiling of MicroRNAs by Quantitative Real-Time PCR: The Good, the Bad, and the Ugly. Dye-Based High-Throughput qPCR in Microfluidic Platform BioMark™. Surface Plasmon Resonance-Based Biosensor Technology for Real-Time Detection of PCR Products. Current Innovations in the Development and Application of the qPCR in a Resource-Limited Setting. A Review of Isothermal Nucleic Acid Amplification Technologies. Proximity Ligation Assay for Protein Quantification. High-Resolution Melt Analysis. Index.