PCR Strategies

ISBN-13: 9780123721822 / Angielski / Twarda / 1995 / 373 str.

Opis

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both

• Concepts explained
• Methods detailed
• Trouble-shooting emphasized
• Novel applications highlighted
• Key concepts for PCR
• Analysis of PCR products
• Research applications
• Alternative amplification strategies

Spis treści

Principles of PCR Amplification: Preface
The Use of Co-Solvents to Enhance Amplification By the Polymerase Chain Reaction
DNA Polymerase Fidelity: Misinsertion and Mismatch Extension
Extraction of Nucleic Acids: Sample Preparation from Paraffin-Embedded Tissues
Thermostable DNA Polymerases
Direct RNA Amplification
Nucleic Acid Hybridization and Unconventional Bases
Molecular Phylogentic Analysis
The Dutp-Uracil Glycosylase System for Caryover Control and Enhanced Specificity
Quantitation. Analysis of PCR Products: Carrier Detection of Cystic Fibrosis Mutations Using PCR-Amplified DNA and a Mismatch-Binding Protein, Muts
Single-Stranded Conformational Polymorphisms (SSCP)
Analysis of PCR Products By Covalent Reverse Dot Blot Hybridization. HPLC Analysis of PCR Products
Rare Mutation Detection. Heteroduplex Mobility Shift Assays for Phylogenetic Analysis
Considerations for Use of RNA: Amplified DNA Hybrid Detection Using a Monoclonal Antibody Conjugate
PCR Amplification of Vntrs
Site-Specific Mutagenesis Using the Polymerase Chain Reaction. Exact Quantification of DNA/RNA Copy Numbers By PCR/TGGE. PCR in Situ: Amplification and Detection of DNA in a Cellular Context
Chromosome Specific PCR: Maternal Blood
DNA and RNA Fingerprinting Using Arbitrarily Primed PCR
PCR-Based Screening of Yeast Artificial Chromosome (YAC) Libraries
Oligonucleotide Ligands that Discriminate Between Theophylline and Caffeine
Generation of Single Chain Antibody Fragments By PCR. Longer PCR Amplifications
Rapid Screening for Mutations Using Intercalating Dyes
Direct Analysis of Specific Bands from Arbitrarily Primed PCR Reaction
Detection of Leber's Hereditary Optic Neuropathy By Nonradioactive-LCR
Detection of Listeria Monocytogenes By PCR-Coupled Ligase Chain Reaction (LCR)
3SR as an Amplification Strategy for RNA