Organ Regeneration: Methods and Protocols

ISBN-13: 9781493960088 / Angielski / Miękka / 2016 / 377 str.

Joydeep Basu; John W. Ludlow

Opis

This Methods in Molecular Biology (TM) book covers cell isolation and characterization, preparation of biomaterial scaffolding, implantation of regenerative constructs in experimental animals, molecular and histological methods for evaluating results and more.

Spis treści

1. Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering

            Benjamin M. Buehrer and Bentley and Cheatham

 

2. Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs

            Darell W. McCoy

 

3. Isolation of Urothelial Cells from Bladder Tissue

            Namrata Sangha

 

4. Isolation of Pulsatile Cell Bodies from Esophageal Tissue

            John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, and Namrata Sangha

 

5. Cell Isolation Through Whole Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis

            Sonya O. Meheux

 

6. Ex Vivo Culture and Separation of Functional Renal Cells

            Andy Bruce, Kelly Guthrie, and Rusty Kelley

 

7. Isolation and Myogenic Differentiation of Mesenchymal Stem Cells (MSC) for Urologic Tissue Engineering

            Rongpei Wu, Guihua Liu, Shantaram Bharadwaj, and Yuanyuan Zhang

 

8. Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells

            Tori Sampsell-Barron

 

9. Reverse Engineering Life: Physical and Chemical Mimetics for Controlled Stem Cell Differentiation into Cardiomyocytes

            Gary R. Skuse and Kathleen A. Lamkin-Kennard

 

10. Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine

            Teresa Bora Burnette and Andrew T. Bruce

 

11. Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications

            Monica A. Serban, Toyin A. Knight, and Richard G. Payne

 

12. Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

            Adelola O. Oseni, Peter E. Butler, and Alexander M. Seifalian

 

13. Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features

            Scott Rapoport

 

14. Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering

            Richard G. Payne and Toyin A. Knight

 

15. Characterization of a PGA Based Scaffold for Use in a Tissue Engineered Neo-Urinary Conduit

            Toyin A. Knight and Richard G. Payne

 

16. Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications

            Kelly Guthrie, Namrata Sangha, Christopher Genheimer, Joydeep Basu, and John W. Ludlow

 

17. Care of Rodent Models used for Pre-Clinical Evaluation of Tissue Engineered/Regenerative Medicine Product Candidates

            Kim L. Mihalko

 

18. Bioreactor Design Considerations for Hollow Organs

            Jeff Fish, Craig Halberstadt, Darell McCoy, and Neil Robbins

 

19. Construction of a Multicoaxial Hollow Fiber Bioreactor

            Randall McClelland, Katherine Tech, and Jeffrey M. Macdonald

 

20. Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas

            Emmanuel C. Opara, John P. McQuilling, and Alan C. Farney

 

21. Bioengineered Skin Substitutes

            Pedro Lei, Hui You, and Stelios T. Andreadis

 

22. Formulation of Selected Renal Cells for Implantation into a Kidney

            Craig Halberstadt, Neil Robbins, Darell McCoy, Kelly Guthrie, Andrew Bruce, Toyin Knight, and Richard Payne

 

23. Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold

            Pedro M. Baptista, Dipen Vyas, Emma Moran, Zhan Wang, and Shay Soker

 

24. A "Living Bioreactor" for the Production of Tissue Engineered Small Intestine

            Daniel E. Levin, Frederic G. Sala, Erik R. Barthel, Allison L. Speer, Xiaogang Hou, Yasuhiro Torashima, and Tracy C. Grikscheit

 

25. Tissue Engineering of Esophagus and Small Intestine in Rodent Injury Models

            Joydeep Basu, Kim Mihalko, Elias Rivera, Kelly I. Guthrie, Christopher Genheimer, Namrata Sangha, and John W. Ludlow

 

26. Scanning Electron Microscopy Evaluation of Endothelialized Tissue Engineered Constructs

            Sandra L. Johnson

 

27. Genotypic and Phenotypic Analysis of in vivo Tissue Regeneration in an Animal Model

            Christopher W. Genheimer

 

28. Histological Evaluation of Tissue Regeneration Using Biodegradable Scaffold Seeded by Autologous Cells for Tubular/Hollow Organs Applications

            Elias A. Rivera and Manuel J. Jayo

Blurb

Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response to the growing interest in organ and tissue regeneration as a means to treat disease. Topics cover methods such as isolation and characterization of cells from selected soft tissues and solid organs, preparation and evaluation of natural and synthetic biomaterial scaffolding, implantation of regenerative constructs within experimental animals, and evaluation of regenerative outcomes by molecular and histological methodologies. Written in the successful Methods in Molecular Biology^TM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Organ Regeneration: Methods and Protocols serves as a detailed guide to aid newcomers and seasoned veterans in their developmental and experimental work in tissue engineering and regenerative medicine.