Oral Biology: Molecular Techniques and Applications

ISBN-13: 9781493961429 / Angielski / Miękka / 2016 / 422 str.

Gregory J. Seymour; Mary P. Cullinan; Nicholas C. K. Heng

Opis

In this book, expert researchers in oral biology provide a selection of in-depth methods and techniques optimized for particular applications, which can be readily adapted to particular organisms or areas of interest.

Recenzje

From the reviews:

"This book comprehensively reviews a wide range of techniques and protocols from diverse areas of oral biology, encompassing current research on microbes, host tissues and saliva. The book is obviously aimed at active researchers in the various fields, but because the research topics are so diverse, I would recommend purchase by institutes and departments ... ." (Adam Roberts, Microbiology Today, May, 2011)

Spis treści

Part I: Saliva Studies 1. Gene Therapy of Salivary Diseases Bruce J. Baum, Janik Adriaansen, Ana P. Cotrim, Corinne M. Goldsmith, Paola Perez, Senrong Qi, Anne M. Rowzee, and Changyu Zheng 2. Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications Bradley Stephen Henson and David T. Wong 3. Proteomic Analyses of Saliva: 2-D Gel Electrophoresis, LC-MS/MS, and Western Blotting Shen Hu, Jiang Jiang, and David T. Wong 4. Transcriptomic Analyses of Saliva Viswanathan Palanisamy and David T. Wong Part II: Oral Microbiology 5. The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniques José F. Siqueira, Jr. and Isabela N. Rôças 6. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE) José F. Siqueira, Jr., Mitsuo Sakamoto, and Alexandre S. Rosado 7. Protocols to Study the Physiology of Oral Biofilms José A. Lemos, Jacqueline Abranches, Hyun Koo, Robert E. Marquis, and Robert A. Burne 8. Adhesion of Yeast and Bacteria to Oral Surfaces Richard D. Cannon, Karl M. Lyons, Kenneth Chong, and Ann R. Holmes 9. Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction Stephen M. Hamlet 10. Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopy Abdul Aziz, Dikesh Parmar, Andrew McNaughton, and Geoffrey R. Tompkins 11. Characterization of Anti-Competitor Activities Produced by Oral Bacteria Fengxia Qi and Jens Kreth 12. Natural Transformation of Oral Streptococci Fernanda Cristina Petersen and Anne Aamdal Scheie 13. Use of In Vivo Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalis Shannon M. Wallet, Jin Chung, and Martin Handfield 14. Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX System Nicholas C.K. Heng and Jo-Ann L. Stanton 15. Useof a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps Erwin Lamping and Richard D. Cannon Part III: Cells and Tissues 16. Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expression Katja Närhi and Irma Thesleff 17. A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells Krzysztof Mrozik, Stan Gronthos, Songtao Shi, and P. Mark Bartold 18. Preclinical Methods for the Evaluation of Periodontal Regeneration In vivo Yang-Jo Seol, Gaia Pellegrini, Lea M. Franco, Po-Chun Chang, Chan-Ho Park, and William V. Giannobile 19. Proteomic Analysis of Dental Tissue Microsamples Jonathan E. Mangum, Jew C. Kon, and Michael J. Hubbard 20. Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistry Pauline J. Ford 21. Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogens Koichi Tabeta and Kazuhisa Yamazaki 22. Single-Strand Conformation Polymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Disease Kazuhisa Yamazaki and Harue Ito 23. Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts Patty Chou and Trudy J. Milne 24. The Use of Gene Arrays in Deciphering the Pathobiology of Periodontal Diseases Moritz Kebschull and Panos N. Papapanou 25. Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Software Ryan T. Demmer, Paul Pavlidis, and Panos N. Papapanou

Blurb

With so many major advances over the past two decades coming through the application of molecular biology and nanotechnology, it is essential that dental research, education, and practice keep pace with the rapid progress of science. In Oral Biology: Molecular Techniques and Applications, expert researchers in the field have provided a selection of in depth methods and techniques optimized for particular applications, which can be readily adapted to particular organisms or areas of interest. Divided into three convenient sections, the book covers the study of saliva, as it is a rich source of biomolecules for study at the molecular level, which may lead to the identification of susceptibility to particular diseases, the study of the microbial inhabitants that share the oral cavity with us, as well as a range of protocols that facilitate assessment of the molecular behavior of oral cells and tissues in health and during disease progression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Oral Biology: Molecular Techniques and Applications is an ideal resource not only to the new researcher but also to the seasoned laboratory veteran including cell biologists, microbiologists, and any researchers intent on delving into the vital world of oral biology.