1.
Optogenetics:
Basic Concepts and Their Development Yong Ku Cho and Dan Li 2.
Natural
Resources for Optogenetic Tools Tilo Mathes 3.Algal Photobiology: A Rich Source of
Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics Arash Kianianmomeni and Armin
Hallmann 4.Reversible Photoregulation of gene
Expression and Translation Shinzi Ogasawara 5.Controlling Protein Activity and
Degradation Using Blue-Light Anne P. Lutz, Christian Renicke, and
Christof Taxis 6.Photo-Control of Protein Function Using
Photoactive Yellow Protein Jakeb M. Reis and G. Andrews Woolley 7. A Fluorometric Activity Assay for
Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators Charlotte Helene Chumacher, Heinz G.
Körschen, Christopher Nicol, Carlos Gasser, Reinhard
Seifert, Martin Schwärzel, and Andreas Möglich 8.Optogenetic Control of Pancreatic Islets Thomas M. Reinbothe and Inês G.
Mollet 9.Optogenetics in Plants: Red/Far-Red Light
Control of Gene Expression Rocio Ochoa-Fernandez, Sophia L.
Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Müller, Wilfried Weber, and
Matias D. Zurbriggen 10.Perfect Channelrhodopsins: A User Guide Jonas Wietek and Matthias Prigge 11.Optogenetics in Drosophila Neuroscience Thomas Riemensperger, Robert J.
Kittel, and André Fiala 12.Optogenetic Control of Mammalian Ion
Channels with Chemical Photoswitches Damien Lemoine, Romain Durand-de
Cuttoli, and Alexandre Mourot 13.Optogenetic Modulation of Locomotor
Activity on Free Behaving Rats Kedi Xu, Jiacheng Zhang, Songchao
Guo, and Xiaoxiang Zheng 14.Combines Optogenetic and Chemogenetic
Control of Neurons Ken Berglund, Jack K. Tung, Bryan
Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender 15.Intracranial Injection of an Optogenetics
Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in
Rat Brain Cortex Christopher Pawela, Edgar DeYoe, and
Ramin Pashaie 16.An Optomized Calcium-Phosphate Transfection
Method for Characterizing Genetically Encoded Tools in Primary Neurons Shiyao Wang and Yong Ku Cho
17.Optogenetic Approaches for Mesoscopic Brain
Mapping Michael Kyweriga and Majid H.
Mohajerani 18.Optogenetic Tools for Confined Stimulation
in Deep Brain Structures Alexandre Castonguay, Sébastien
Thomas, Frédéric Lesage, and Christian Casanova 19.Remote Patterning of Transgene Expression
Using Near Infrared-Responsive Plasmonic Hydrogels Francisco Martín-Saavedra and Nuria
Vilaboa 20.Optogenetic Light Crafting Tools for the
Control of Cardiac Arrhythmias Claudia Richter, Jan Christoph,
Stephan E. Lehnart, and Stefan Luther 21.Inscribing Optocal Excitability to
Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary
Cardiac Fibroblasts Jinzhu Yu and Emilia Entcheva 22.Optogenetic Engineering of Atrial
Cardiomyocytes Iolanda Feola, Alexander Teplenin,
Antoine A.F. de Vries, and Daniël A. Pijnappels 23. A Multi-Channel Recording System with
Optical Stimulation for Closed-Loop Optogenetic Experiments Carmen Bartic, Francesco P.
Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova 24.Optogenetic Control of Fibroblast Growth
Factor Receptor Signaling Nury Kim, Jin Man Kim, and Won Do Heo 25. Protein Inactivation by Optogenetic
Trapping in Living Cells Hyerim Park, Sangkyu Lee, and Won Do
Heo 26.Optogenetic Manipulation of Selective
Neural Activity in Free-Moving Drosophila
Adults Po-Yen Hsiao, Ming-Chin Wu, Yen-Yin
Lin, Chein-Chung Fu, and Ann-Shyn Chiang 27.Guidelines for Photoreceptor Engineering Thea Ziegler, Charlotte Helene
Schumacher, and Andreas Möglich
This volume provides researchers with up-to-date protocols and takes a
close look at current research and promising applications. Optogenetics:
Methods and Protocols contains a collection of recently developed technical
protocols on optogenetic applications in neuroscience, brain mapping, treatment
of neurological disorders, and restoration of visual function. Several
introductory and discussion chapters offer a wide overview about sources and
diversity of optogenetic tools, design strategies, and potential applications
in other fields like plant research. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers
an interdisciplinary view of optogenetic applications and its potential to
develop as a valuable tool for basic research, as well as biomedical and
biotechnological applications.