Optogenetics: Methods and Protocols

ISBN-13: 9781493935109 / Angielski / Twarda / 2016 / 408 str.

Arash Kianianmomeni

Opis

This volume provides researchers with up-to-date protocols and takes aclose look at current research and promising applications.

Spis treści

1.       Optogenetics: Basic Concepts and Their Development
Yong Ku Cho and Dan Li

2.       Natural Resources for Optogenetic Tools
Tilo Mathes

3.       Algal Photobiology: A Rich Source of Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics
Arash Kianianmomeni and Armin Hallmann

4.       Reversible Photoregulation of gene Expression and Translation
Shinzi Ogasawara

5.       Controlling Protein Activity and Degradation Using Blue-Light
Anne P. Lutz, Christian Renicke, and Christof Taxis

6.       Photo-Control of Protein Function Using Photoactive Yellow Protein
Jakeb M. Reis and G. Andrews Woolley

7.       A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators
Charlotte Helene Chumacher, Heinz G. Körschen, Christopher Nicol, Carlos Gasser, Reinhard Seifert, Martin Schwärzel, and Andreas Möglich

8.       Optogenetic Control of Pancreatic Islets
Thomas M. Reinbothe and Inês G. Mollet

9.       Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Rocio Ochoa-Fernandez, Sophia L. Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Müller, Wilfried Weber, and Matias D. Zurbriggen

10.   Perfect Channelrhodopsins: A User Guide
Jonas Wietek and Matthias Prigge

11.   Optogenetics in Drosophila Neuroscience
Thomas Riemensperger, Robert J. Kittel, and André Fiala

12.   Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
Damien Lemoine, Romain Durand-de Cuttoli, and Alexandre Mourot

13.   Optogenetic Modulation of Locomotor Activity on Free Behaving Rats
Kedi Xu, Jiacheng Zhang, Songchao Guo, and Xiaoxiang Zheng

14.   Combines Optogenetic and Chemogenetic Control of Neurons
Ken Berglund, Jack K. Tung, Bryan Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender
15.   Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
Christopher Pawela, Edgar DeYoe, and Ramin Pashaie

16.   An Optomized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
Shiyao Wang and Yong Ku Cho

17.   Optogenetic Approaches for Mesoscopic Brain Mapping
Michael Kyweriga and Majid H. Mohajerani

18.   Optogenetic Tools for Confined Stimulation in Deep Brain Structures
Alexandre Castonguay, Sébastien Thomas, Frédéric Lesage, and Christian Casanova

19.   Remote Patterning of Transgene Expression Using Near Infrared-Responsive Plasmonic Hydrogels
Francisco Martín-Saavedra and Nuria Vilaboa

20.   Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias
Claudia Richter, Jan Christoph, Stephan E. Lehnart, and Stefan Luther

21.   Inscribing Optocal Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts
Jinzhu Yu and Emilia Entcheva

22.   Optogenetic Engineering of Atrial Cardiomyocytes
Iolanda Feola, Alexander Teplenin, Antoine A.F. de Vries, and Daniël A. Pijnappels

23.   A Multi-Channel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments
Carmen Bartic, Francesco P. Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova

24.   Optogenetic Control of Fibroblast Growth Factor Receptor Signaling
Nury Kim, Jin Man Kim, and Won Do Heo

25.   Protein Inactivation by Optogenetic Trapping in Living Cells
Hyerim Park, Sangkyu Lee, and Won Do Heo

26.   Optogenetic Manipulation of Selective Neural Activity in Free-Moving Drosophila Adults
Po-Yen Hsiao, Ming-Chin Wu, Yen-Yin Lin, Chein-Chung Fu, and Ann-Shyn Chiang

27.   Guidelines for Photoreceptor Engineering
Thea Ziegler, Charlotte Helene Schumacher, and Andreas Möglich

Blurb

This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications.